Selective activation of TRPA1 ion channels by nitrobenzene skin sensitizers DNFB and DNCB.
DNCB
DNFB
TRPA1
contact dermatitis
electrophiles
nitrobenzene
skin sensitizer
Journal
The Journal of biological chemistry
ISSN: 1083-351X
Titre abrégé: J Biol Chem
Pays: United States
ID NLM: 2985121R
Informations de publication
Date de publication:
02 2022
02 2022
Historique:
received:
07
08
2021
revised:
17
12
2021
accepted:
28
12
2021
pubmed:
2
1
2022
medline:
30
4
2022
entrez:
1
1
2022
Statut:
ppublish
Résumé
2, 4-dinitrofluorobenzene (DNFB) and 2, 4-dinitrochlorobenzene (DNCB) are well known as skin sensitizers that can cause dermatitis. DNFB has shown to more potently sensitize skin; however, how DNFB and DNCB cause skin inflammation at a molecular level and why this difference in their sensitization ability is observed remain unknown. In this study, we aimed to identify the molecular targets and mechanisms on which DNFB and DNCB act. We used a fluorescent calcium imaging plate reader in an initial screening assay before patch-clamp recordings for validation. Molecular docking in combination with site-directed mutagenesis was then carried out to investigate DNFB and DNCB binding sites in the TRPA1 ion channel that may be selectively activated by these tow sensitizers. We found that DNFB and DNCB selectively activated TRPA1 channel with EC
Identifiants
pubmed: 34973335
pii: S0021-9258(21)01365-X
doi: 10.1016/j.jbc.2021.101555
pmc: PMC8800105
pii:
doi:
Substances chimiques
Dinitrochlorobenzene
0
TRPA1 Cation Channel
0
TRPA1 protein, human
0
Dinitrofluorobenzene
D241E059U6
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
101555Informations de copyright
Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Conflict of interest The authors declare that they have no conflicts of interest with the contents of this article.