3D printed carboxymethyl cellulose scaffolds for autologous growth factors delivery in wound healing.
Animals
Carboxymethylcellulose Sodium
/ chemistry
Diabetes Mellitus, Type 1
Drug Delivery Systems
Intercellular Signaling Peptides and Proteins
/ chemistry
Male
Particle Size
Platelet-Rich Plasma
/ chemistry
Printing, Three-Dimensional
Rats
Rats, Sprague-Dawley
Tissue Scaffolds
/ chemistry
Transforming Growth Factor beta1
/ chemistry
Vascular Endothelial Growth Factors
/ chemistry
Wound Healing
/ drug effects
3D printing
Angiogenesis
Carboxymethyl cellulose
Cell mobility
Platelet rich plasma
Rheological properties
Wound healing
microCT
Journal
Carbohydrate polymers
ISSN: 1879-1344
Titre abrégé: Carbohydr Polym
Pays: England
ID NLM: 8307156
Informations de publication
Date de publication:
15 Feb 2022
15 Feb 2022
Historique:
received:
04
08
2021
revised:
02
10
2021
accepted:
17
11
2021
entrez:
2
1
2022
pubmed:
3
1
2022
medline:
17
3
2022
Statut:
ppublish
Résumé
This work aims to use carboxymethyl cellulose (CMC) as main structural and functional component of 3D printed scaffolds for healing of diabetic wounds. Differently from previous inks involving small contents in CMC, herein sterile (steam-heated) concentrated CMC solely dispersions (10-20%w/v) were screened regarding printability and fidelity properties. CMC (15%w/v)-citric acid inks showed excellent self-healing rheological properties and stability during storage. CMC scaffolds loaded with platelet rich plasma (PRP) sustained the release of relevant growth factors. CMC scaffolds both with and without PRP promoted angiogenesis in ovo, stem cell migration in vitro, and wound healing in a diabetic model in vivo. Transparent CMC scaffolds allowed direct monitoring of bilateral full-thickness wounds created in rat dorsum. CMC scaffolds facilitated re-epithelialization, granulation, and angiogenesis in full-thickness skin defects, and the performance was improved when combined with PRP. Overall, CMC is pointed out as outstanding component of active dressings for diabetic wounds.
Identifiants
pubmed: 34973742
pii: S0144-8617(21)01311-4
doi: 10.1016/j.carbpol.2021.118924
pii:
doi:
Substances chimiques
Intercellular Signaling Peptides and Proteins
0
Transforming Growth Factor beta1
0
Vascular Endothelial Growth Factors
0
Carboxymethylcellulose Sodium
K679OBS311
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
118924Informations de copyright
Copyright © 2021. Published by Elsevier Ltd.