Viral vectors for opto-electrode recording and photometry-based imaging of oxytocin neurons in anesthetized and socially interacting rats.

Animals Electrodes Fiber Optic Technology / methods Neurons / physiology Oxytocin Photometry / methods Rats
Behavior Biotechnology and bioengineering Microscopy Model Organisms Neuroscience

Journal

STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501

Informations de publication

Date de publication:
18 03 2022
Historique:
entrez: 3 1 2022
pubmed: 4 1 2022
medline: 9 4 2022
Statut: epublish

Résumé

Here, we present a step-by-step protocol to target, record, and manipulate the activity of oxytocin neurons in awake rats. The protocol includes a procedure to record the activity of oxytocin neurons from awake and socially interacting rats using opto-electrodes for simultaneous electrophysiological recording and virally based cell-type-specific opto-tagging with Channelrhodopsin 2. Furthermore, we illustrate a procedure for optically guided implantation of optic fiber and imaging of oxytocin neuron population activity expressing calcium indicator GCaMP6s with the fiber photometry technique. For complete details on the use and execution of this protocol, please refer to Tang et al., 2020.

Identifiants

DOI: 10.1016/j.xpro.2021.101032 PMID: 34977678 PMC: PMC8683652
pubmed: 34977678
doi: 10.1016/j.xpro.2021.101032
pii: S2666-1667(21)00738-3
pmc: PMC8683652
doi:

Substances chimiques

Oxytocin 50-56-6

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

101032

Informations de copyright

© 2021 The Author(s).

Déclaration de conflit d'intérêts

The authors declare no competing interests.

Références

J Neurosci. 2020 Mar 11;40(11):2282-2295
pubmed: 32024781
Neuron. 2012 Feb 9;73(3):553-66
pubmed: 22325206
Psychoneuroendocrinology. 2019 Aug;106:77-84
pubmed: 30954921
Nature. 2013 Jul 18;499(7458):295-300
pubmed: 23868258
Nature. 2021 Aug;596(7873):553-557
pubmed: 34381215
Neurophotonics. 2019 Jul;6(3):035011
pubmed: 31528656
PLoS One. 2009 Jul 07;4(7):e6099
pubmed: 19584920
Nat Neurosci. 2020 Sep;23(9):1125-1137
pubmed: 32719563

Auteurs

Yan Tang (Y)

Center for Psychiatric Neurosciences, University Hospital of Lausanne (CHUV), 1008 Prilly-Lausanne, Switzerland.

Diego Benusiglio (D)

European Molecular Biology Laboratory, Epigenetics and Neurobiology Unit, 00015 Monterotondo (Rome), Italy.
Italian Institute of Technology, Neuroscience and Behaviour Laboratory, 00161 Rome, Italy.

Arthur Lefevre (A)

Department of Neuropeptide Research in Psychiatry, Central Institute of Mental Health, Medical Faculty Mannheim, University of Heidelberg, J5, 68159 Mannheim, Germany.

Stephanie Küppers (S)

Department of Neuropeptide Research in Psychiatry, Central Institute of Mental Health, Medical Faculty Mannheim, University of Heidelberg, J5, 68159 Mannheim, Germany.

Olga Lapies (O)

Centre National de la Recherche Scientifique and University of Strasbourg, Institute of Cellular and Integrative Neurosciences, INCI CNRS UPR3212, 8, Allée du Général Rouvillois, 67000 Strasbourg, France.

Damien Kerspern (D)

Centre National de la Recherche Scientifique and University of Strasbourg, Institute of Cellular and Integrative Neurosciences, INCI CNRS UPR3212, 8, Allée du Général Rouvillois, 67000 Strasbourg, France.

Alexandre Charlet (A)

Centre National de la Recherche Scientifique and University of Strasbourg, Institute of Cellular and Integrative Neurosciences, INCI CNRS UPR3212, 8, Allée du Général Rouvillois, 67000 Strasbourg, France.

Valery Grinevich (V)

Department of Neuropeptide Research in Psychiatry, Central Institute of Mental Health, Medical Faculty Mannheim, University of Heidelberg, J5, 68159 Mannheim, Germany.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Viral vectors for opto-electrode recording and...
questionsmedicales.fr › Équipement et fournitures › Équipement et fournitures électriques › Électrodes › Viral vectors for opto-electrode recording and...
questionsmedicales.fr › Disciplines des sciences naturelles › Physique › Technologie des fibres optiques › Viral vectors for opto-electrode recording and...
questionsmedicales.fr › Cellules › Neurones › Viral vectors for opto-electrode recording and...
questionsmedicales.fr › Acides aminés, peptides et protéines › Peptides › Hormones peptidiques › Hormones hypophysaires › Hormones post-hypophysaires › Ocytocine › Viral vectors for opto-electrode recording and...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Photométrie › Viral vectors for opto-electrode recording and...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Rats › Viral vectors for opto-electrode recording and...