Evaluation of Cronobacter sakazakii biofilm formation after sdiA knockout in different osmotic pressure conditions.
Biofilm
Cronobacter sakazakii
Extracellular matrix
Motility
Osmotic stress
sdiA
Journal
Food research international (Ottawa, Ont.)
ISSN: 1873-7145
Titre abrégé: Food Res Int
Pays: Canada
ID NLM: 9210143
Informations de publication
Date de publication:
01 2022
01 2022
Historique:
received:
02
09
2021
revised:
23
11
2021
accepted:
02
12
2021
entrez:
4
1
2022
pubmed:
5
1
2022
medline:
28
1
2022
Statut:
ppublish
Résumé
This study characterizes the impact of sdiA on biofilm formation under normal or osmotic stress conditions in Cronobacter sakazakii by constructing a sdiA deletion mutant (ΔsdiA). Here, the downregulation of flagellar assembly-related genes and upregulation of capsular, cellulose and lipopolysaccharide biosynthesis-associated genes in ΔsdiA were observed when compared to the wild type strain (WT) through transcriptomic analysis. Meanwhile, reduced ability of motility, enhanced cell surface hydrophobicity and stronger biofilms with extracellular matrix were observed in WT with deletion of sdiA. Both WT and ΔsdiA formed more biofilm in low osmotic stress medium, while in hyperosmolarity conditions, formation of biofilm was dramatically reduced. Our findings supported that sdiA might suppress biofilm formation of C. sakazakii by regulating biosynthesis of flagellar and extracellular polymeric substances. This study investigates the role of sdiA on biofilm formation in C. sakazakii, and provides the basis for the inhibition of C. sakazakii in food industry and infant-feeding.
Identifiants
pubmed: 34980413
pii: S0963-9969(21)00786-9
doi: 10.1016/j.foodres.2021.110886
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
110886Informations de copyright
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