A segmentation clock patterns cellular differentiation in a bacterial biofilm.
Bacillus subtilis
/ genetics
Biofilms
/ growth & development
Body Patterning
/ genetics
Gene Expression
Gene Expression Regulation, Developmental
Kinetics
Models, Biological
Nitrogen
/ metabolism
Signal Transduction
/ genetics
Somites
/ growth & development
Spores, Bacterial
/ growth & development
Stress, Physiological
/ genetics
Time Factors
Bacillus subtilis
biofilm
clock and wavefront
multicellularity
nitrogen stress response
pattern formation
segmentation clock
somitogenesis
sporulation
Journal
Cell
ISSN: 1097-4172
Titre abrégé: Cell
Pays: United States
ID NLM: 0413066
Informations de publication
Date de publication:
06 01 2022
06 01 2022
Historique:
received:
09
06
2021
revised:
13
10
2021
accepted:
30
11
2021
entrez:
7
1
2022
pubmed:
8
1
2022
medline:
23
2
2022
Statut:
ppublish
Résumé
Contrary to multicellular organisms that display segmentation during development, communities of unicellular organisms are believed to be devoid of such sophisticated patterning. Unexpectedly, we find that the gene expression underlying the nitrogen stress response of a developing Bacillus subtilis biofilm becomes organized into a ring-like pattern. Mathematical modeling and genetic probing of the underlying circuit indicate that this patterning is generated by a clock and wavefront mechanism, similar to that driving vertebrate somitogenesis. We experimentally validated this hypothesis by showing that predicted nutrient conditions can even lead to multiple concentric rings, resembling segments. We additionally confirmed that this patterning mechanism is driven by cell-autonomous oscillations. Importantly, we show that the clock and wavefront process also spatially patterns sporulation within the biofilm. Together, these findings reveal a biofilm segmentation clock that organizes cellular differentiation in space and time, thereby challenging the paradigm that such patterning mechanisms are exclusive to plant and animal development.
Identifiants
pubmed: 34995513
pii: S0092-8674(21)01404-5
doi: 10.1016/j.cell.2021.12.001
pmc: PMC8754390
mid: NIHMS1765041
pii:
doi:
Substances chimiques
Nitrogen
N762921K75
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
145-157.e13Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM121888
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM139645
Pays : United States
Informations de copyright
Copyright © 2021 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests The authors declare no competing interests.
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