The effect of oral iron supplementation on the gut microbiota, gut inflammation, and iron status in iron-depleted South African school-age children with virally suppressed HIV and without HIV.
Gut inflammation
Gut microbiota
HIV
Iron deficiency
Iron intervention
School-age children
Journal
European journal of nutrition
ISSN: 1436-6215
Titre abrégé: Eur J Nutr
Pays: Germany
ID NLM: 100888704
Informations de publication
Date de publication:
Jun 2022
Jun 2022
Historique:
received:
17
10
2021
accepted:
20
12
2021
pubmed:
9
1
2022
medline:
18
5
2022
entrez:
8
1
2022
Statut:
ppublish
Résumé
Both HIV and oral iron interventions may alter gut microbiota composition and increase gut inflammation. We determined the effect of oral iron supplementation on gut microbiota composition, gut inflammation, and iron status in iron-depleted South Africa school-aged children living with HIV (HIV+) but virally suppressed on antiretroviral therapy and children without HIV (HIV-ve). In this before-after intervention study with case-control comparisons, we provided 55 mg elemental iron from ferrous sulphate, once daily for 3 months, to 33 virally suppressed (< 50 HIV RNA copies/mL) HIV+ and 31 HIV-ve children. At baseline and endpoint, we assessed microbial composition of faecal samples (16S rRNA sequencing), and markers of gut inflammation (faecal calprotectin), anaemia (haemoglobin) and iron status (plasma ferritin, soluble transferrin receptor). This study was nested within a larger trial registered at clinicaltrials.gov as NCT03572010. HIV+ (11.3y SD ± 1.8, 46% male) and HIV-ve (11.1y SD ± 1.7, 52% male) groups did not significantly differ in age or sex ratio. Following iron supplementation, improvements were observed in haemoglobin (HIV+ : 118 to 124 g/L, P = 0.003; HIV-ve: 120 to 124 g/L, P = 0.003), plasma ferritin (HIV+ : 15 to 34 µg/L, P < 0.001; HIV-ve: 18 to 37 µg/L, P < 0.001), and soluble transferrin receptor (HIV+ : 7.1 to 5.9 mg/L, P < 0.001; HIV-ve: 6.6 to 5.7 mg/L, P < 0.001), with no significant change in the relative abundance of any genera, alpha diversity of the gut microbiota (HIV+ : P = 0.37; HIV-ve: P = 0.77), or faecal calprotectin (HIV+ : P = 0.42; HIV-ve: P = 0.80). Our findings suggest that oral iron supplementation can significantly improve haemoglobin and iron status without increasing pathogenic gut microbial taxa or gut inflammation in iron-depleted virally suppressed HIV+ and HIV-ve school-age children.
Identifiants
pubmed: 34997267
doi: 10.1007/s00394-021-02793-9
pii: 10.1007/s00394-021-02793-9
doi:
Substances chimiques
Hemoglobins
0
Leukocyte L1 Antigen Complex
0
RNA, Ribosomal, 16S
0
Receptors, Transferrin
0
Ferritins
9007-73-2
Iron
E1UOL152H7
Banques de données
ClinicalTrials.gov
['NCT03572010']
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2067-2078Subventions
Organisme : Thrasher Research Fund
ID : 14199
Organisme : Fondation L'Oréal
ID : L'Oréal-UNESCO For Women in Science Sub-Saharan Africa Fellowship
Organisme : Ernst and Ethel Eriksen Trust
ID : Bursary
Organisme : South African Medical Research Council
ID : Bongani Mayosi National Health Scholars Programme
Informations de copyright
© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany.
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