Mobility of kinetochore proteins measured by FRAP analysis in living cells.
CCAN
CENP-C
Kinetochore
Mis12 complex
Journal
Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology
ISSN: 1573-6849
Titre abrégé: Chromosome Res
Pays: Netherlands
ID NLM: 9313452
Informations de publication
Date de publication:
03 2022
03 2022
Historique:
received:
11
10
2021
accepted:
23
11
2021
revised:
22
11
2021
pubmed:
9
1
2022
medline:
12
4
2022
entrez:
8
1
2022
Statut:
ppublish
Résumé
The kinetochore is essential for faithful chromosome segregation during mitosis and is assembled through dynamic processes involving numerous kinetochore proteins. Various experimental strategies have been used to understand kinetochore assembly processes. Fluorescence recovery after photobleaching (FRAP) analysis is also a useful strategy for revealing the dynamics of kinetochore assembly. In this study, we introduced fluorescence protein-tagged kinetochore protein cDNAs into each endogenous locus and performed FRAP analyses in chicken DT40 cells. Centromeric protein (CENP)-C was highly mobile in interphase, but immobile during mitosis. CENP-C mutants lacking the CENP-A-binding domain became mobile during mitosis. In contrast to CENP-C, CENP-T and CENP-H were immobile during both interphase and mitosis. The mobility of Dsn1, which is a component of the Mis12 complex and directly binds to CENP-C, depended on CENP-C mobility during mitosis. Thus, our FRAP assays provide dynamic aspects of how the kinetochore is assembled.
Identifiants
pubmed: 34997387
doi: 10.1007/s10577-021-09678-x
pii: 10.1007/s10577-021-09678-x
pmc: PMC8942963
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
43-57Informations de copyright
© 2021. The Author(s).
Références
J Cell Biol. 2006 Apr 10;173(1):9-17
pubmed: 16585270
EMBO J. 2021 Mar 1;40(5):e105671
pubmed: 33463726
Cell. 2008 Dec 12;135(6):1039-52
pubmed: 19070575
J Biophotonics. 2008 Aug;1(3):245-54
pubmed: 19412974
EMBO J. 2001 Aug 15;20(16):4603-17
pubmed: 11500386
Cell. 2006 Dec 1;127(5):983-97
pubmed: 17129783
Prog Mol Subcell Biol. 2017;56:29-57
pubmed: 28840232
EMBO J. 1999 Aug 2;18(15):4196-209
pubmed: 10428958
Cell. 2006 Dec 1;127(5):969-82
pubmed: 17129782
Cell Mol Life Sci. 2020 Aug;77(15):2981-2995
pubmed: 32052088
Genes Cells. 2006 Jun;11(6):673-84
pubmed: 16716197
Nat Commun. 2017 Jun 09;8:15775
pubmed: 28598437
J Cell Biol. 2008 Mar 24;180(6):1101-14
pubmed: 18347072
Nature. 2010 Oct 14;467(7317):805-10
pubmed: 20944740
J Cell Biol. 2009 Jul 27;186(2):173-82
pubmed: 19620631
Nature. 2014 Oct 30;514(7524):646-9
pubmed: 25156254
J Cell Biol. 2019 Dec 2;218(12):4042-4062
pubmed: 31676716
Dev Cell. 2014 Sep 8;30(5):496-508
pubmed: 25203206
Nature. 2016 Sep 8;537(7619):249-253
pubmed: 27580032
Cell. 2012 Feb 3;148(3):487-501
pubmed: 22304917
Science. 2013 May 31;340(6136):1110-3
pubmed: 23723239
Nat Biotechnol. 2013 Sep;31(9):827-32
pubmed: 23873081
Nat Rev Mol Cell Biol. 2016 Jan;17(1):16-29
pubmed: 26601620
Curr Opin Cell Biol. 2018 Jun;52:73-81
pubmed: 29477052
Nat Methods. 2012 Jun 28;9(7):676-82
pubmed: 22743772
Curr Opin Struct Biol. 2016 Apr;37:152-63
pubmed: 27039078
Science. 2015 May 8;348(6235):699-703
pubmed: 25954010
Cell. 2016 Nov 3;167(4):1028-1040.e15
pubmed: 27881301
J Cell Biol. 2015 Jul 6;210(1):11-22
pubmed: 26124289
Cell. 1992 Jul 10;70(1):115-25
pubmed: 1339310
Nat Methods. 2017 Jan;14(1):53-56
pubmed: 27869816
Nat Cell Biol. 2018 Dec;20(12):1378-1388
pubmed: 30420662
Nat Cell Biol. 2006 May;8(5):458-69
pubmed: 16622419
Mol Biol Cell. 2007 Jun;18(6):2155-68
pubmed: 17392512
Hum Mol Genet. 1997 Dec;6(13):2301-8
pubmed: 9361037
Nat Cell Biol. 2013 Sep;15(9):1056-66
pubmed: 23873148
Nat Struct Mol Biol. 2016 Mar;23(3):204-208
pubmed: 26878239
Open Biol. 2014 Feb 12;4:130229
pubmed: 24522885
Chromosoma. 2016 Sep;125(4):645-59
pubmed: 27376724
Mol Biol Cell. 2015 Nov 1;26(21):3768-76
pubmed: 26354420
Nat Cell Biol. 2006 May;8(5):446-57
pubmed: 16622420