Detailed epitope mapping of SARS-CoV-2 nucleoprotein reveals specific immunoresponse in cats and dogs housed with COVID-19 patients.
COVID19
Double antigen ELISA
Epitope mapping
SARS-CoV-2
SARS-CoV-2 nucleoprotein
Journal
Research in veterinary science
ISSN: 1532-2661
Titre abrégé: Res Vet Sci
Pays: England
ID NLM: 0401300
Informations de publication
Date de publication:
Mar 2022
Mar 2022
Historique:
received:
27
07
2021
revised:
01
12
2021
accepted:
28
12
2021
pubmed:
9
1
2022
medline:
23
2
2022
entrez:
8
1
2022
Statut:
ppublish
Résumé
Since the initial emergence in December 2019, the novel Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has been reported in over 200 countries, representing an unprecedented challenge related to disease control worldwide. In this context, cases of human to animal transmission have been reported, raising concern about the potential role of companion animals in the pandemic and stressing the need for reliable animal testing. In the study, a detailed epitope mapping of SARS-CoV-2 nucleoprotein, using both human and pet sera, allowed the identification of the most antigenic region in the C-terminus domain of the protein, which was used to develop an experimental double antigen-based ELISA. A panel of pre-pandemic sera and sera of animals immunized against (or naturally infected with) related coronaviruses was used to assess assay specificity at 99.5%. Positive sera belonging to animals housed with COVID-19 patients were confirmed with the experimental double-antigen ELISA using Plaque Reduction Neutralization test (PRNT) test as gold standard. The availability of a serological assay that targets a highly specific viral antigen represents a valuable tool for multispecies monitoring of Coronavirus Disease 2019 (COVID-19) infection in susceptible animals.
Identifiants
pubmed: 34998208
pii: S0034-5288(21)00364-7
doi: 10.1016/j.rvsc.2021.12.020
pmc: PMC8723757
pii:
doi:
Substances chimiques
Antibodies, Viral
0
Coronavirus Nucleocapsid Proteins
0
Phosphoproteins
0
nucleocapsid phosphoprotein, SARS-CoV-2
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
81-87Informations de copyright
Copyright © 2022 Elsevier Ltd. All rights reserved.
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