Designer DNA nanostructures for viral inhibition.
Journal
Nature protocols
ISSN: 1750-2799
Titre abrégé: Nat Protoc
Pays: England
ID NLM: 101284307
Informations de publication
Date de publication:
02 2022
02 2022
Historique:
received:
23
03
2021
accepted:
29
09
2021
pubmed:
12
1
2022
medline:
24
2
2022
entrez:
11
1
2022
Statut:
ppublish
Résumé
Emerging viral diseases can substantially threaten national and global public health. Central to our ability to successfully tackle these diseases is the need to quickly detect the causative virus and neutralize it efficiently. Here we present the rational design of DNA nanostructures to inhibit dengue virus infection. The designer DNA nanostructure (DDN) can bind to complementary epitopes on antigens dispersed across the surface of a viral particle. Since these antigens are arranged in a defined geometric pattern that is unique to each virus, the structure of the DDN is designed to mirror the spatial arrangement of antigens on the viral particle, providing very high viral binding avidity. We describe how available structural data can be used to identify unique spatial patterns of antigens on the surface of a viral particle. We then present a procedure for synthesizing DDNs using a combination of in silico design principles, self-assembly, and characterization using gel electrophoresis, atomic force microscopy and surface plasmon resonance spectroscopy. Finally, we evaluate the efficacy of a DDN in inhibiting dengue virus infection via plaque-forming assays. We expect this protocol to take 2-3 d to complete virus antigen pattern identification from existing cryogenic electron microscopy data, ~2 weeks for DDN design, synthesis, and virus binding characterization, and ~2 weeks for DDN cytotoxicity and antiviral efficacy assays.
Identifiants
pubmed: 35013618
doi: 10.1038/s41596-021-00641-y
pii: 10.1038/s41596-021-00641-y
pmc: PMC8852688
mid: NIHMS1771255
doi:
Banques de données
figshare
['10.6084/m9.figshare.c.5409411']
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
282-326Subventions
Organisme : NIAID NIH HHS
ID : R01 AI159454
Pays : United States
Organisme : NIAAA NIH HHS
ID : U01 AA029348
Pays : United States
Informations de copyright
© 2022. The Author(s), under exclusive licence to Springer Nature Limited.
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