Anatomical and hormonal factors determining the development of haploid and zygotic embryos of oat (Avena sativa L.).
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
11 01 2022
11 01 2022
Historique:
received:
08
10
2021
accepted:
23
12
2021
entrez:
12
1
2022
pubmed:
13
1
2022
medline:
25
2
2022
Statut:
epublish
Résumé
A critical step in the production of doubled haploids is a conversion of the haploid embryos into plants. Our study aimed to recognize the reasons for the low germination rate of Avena sativa haploid embryos obtained by distant crossing with maize. Oat cultivars of 'Krezus' and 'Akt' were investigated regarding embryo anatomy, the endogenous phytohormone profiles, and antioxidant capacity. The zygotic embryos of oat were used as a reference. It was found that twenty-one days old haploid embryos were smaller and had a less advanced structure than zygotic ones. Morphology and anatomy modifications of haploid embryos were accompanied by extremely low levels of endogenous auxins. Higher levels of cytokinins, as well as tenfold higher cytokinin to auxin ratio in haploid than in zygotic embryos, may suggest an earlier stage of development of these former. Individual gibberellins reached higher values in 'Akt' haploid embryos than in the respective zygotic ones, while the differences in both types of 'Krezus' embryos were not noticed. Additionally to the hormonal regulation of haploid embryogenesis, the poor germination of oat haploid embryos can be a result of the overproduction of reactive oxygen species, and therefore higher levels of low molecular weight antioxidants and stress hormones.
Identifiants
pubmed: 35017602
doi: 10.1038/s41598-021-04522-y
pii: 10.1038/s41598-021-04522-y
pmc: PMC8752813
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
548Subventions
Organisme : Polish Ministry of Science and Higher Education
ID : NR 3/5457/E-189/M/2017
Informations de copyright
© 2022. The Author(s).
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