Genome-wide analysis of the SWEET gene family and its response to powdery mildew and leaf spot infection in the common oat (Avena sativa L.).
Bioinformatics analysis
Leaf spot
Oat
Powdery mildew
SWEET sugar transporter protein
Journal
BMC genomics
ISSN: 1471-2164
Titre abrégé: BMC Genomics
Pays: England
ID NLM: 100965258
Informations de publication
Date de publication:
24 Oct 2024
24 Oct 2024
Historique:
received:
07
05
2024
accepted:
22
10
2024
medline:
25
10
2024
pubmed:
25
10
2024
entrez:
25
10
2024
Statut:
epublish
Résumé
The nutritional quality and yield of oats (Avena sativa) are often compromised by plant diseases such as red leaf, powdery mildew, and leaf spot. Sugars Will Eventually be Exported Transporters (SWEETs) are newly identified sugar transporters involved in regulating plant growth and stress responses. However, the roles of SWEET genes in biotic stress responses remain uncharacterized in oats. In this study, 13 AsSWEET genes were identified across nine chromosomes of the oat genome, all of which were predicted to contain seven transmembrane regions. Phylogenetic analysis revealed four clades of AsSWEET proteins, with high homology to SWEET proteins in the Poaceae family. Collinearity analysis demonstrated strong relationships between oat and Zea mays SWEETs. Using subcellular localization prediction tools, AsSWEET proteins were predicted to localize to the plasma membrane. Promoter analysis revealed cis-acting elements associated with light response, growth, and stress regulation. Six AsSWEET proteins were predicted to interact in a network centered on AsSWEET1a and AsSWEET11. Gene expression analysis of two oat varieties, 'ForagePlus' and 'Molasses', indicated significant expression differences in several AsSWEET genes following infection with powdery mildew or leaf spot, including AsSWEET1a, AsSWEET1b, AsSWEET2b, AsSWEET3a, AsSWEET11, and AsSWEET16. These SWEET genes are potential candidates for disease resistance in oats. This study provides a foundation for understanding the regulatory mechanisms of AsSWEET genes, particularly in response to powdery mildew and leaf spot, and offers insights for enhancing oat molecular breeding.
Identifiants
pubmed: 39448896
doi: 10.1186/s12864-024-10933-8
pii: 10.1186/s12864-024-10933-8
doi:
Substances chimiques
Plant Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
995Subventions
Organisme : China National Center of Pratacultural Technology Innovation (under preparation) Special Fund for Innovation Platform Construction
ID : CCPTZX2023B05
Organisme : China National Center of Pratacultural Technology Innovation (under preparation) Special Fund for Innovation Platform Construction
ID : CCPTZX2023B05
Organisme : China National Center of Pratacultural Technology Innovation (under preparation) Special Fund for Innovation Platform Construction
ID : CCPTZX2023B05
Organisme : China National Center of Pratacultural Technology Innovation (under preparation) Special Fund for Innovation Platform Construction
ID : CCPTZX2023B05
Organisme : National Natural Science Foundation of China
ID : 32360342, 32301491
Organisme : National Natural Science Foundation of China
ID : 32360342, 32301491
Organisme : Chief Scientist and Program in Gansu Province ,China
ID : 23ZDKA013
Organisme : Chief Scientist and Program in Gansu Province ,China
ID : 23ZDKA013
Informations de copyright
© 2024. The Author(s).
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