Extracellular production of azurin from Pseudomonas aeruginosa in the presence of Triton X-100 or Tween 80.
Azurin
Extracellular secretion
Pseudomonas aeruginosa
Triton X-100
Tween 80
Journal
Bioprocess and biosystems engineering
ISSN: 1615-7605
Titre abrégé: Bioprocess Biosyst Eng
Pays: Germany
ID NLM: 101088505
Informations de publication
Date de publication:
Mar 2022
Mar 2022
Historique:
received:
25
08
2021
accepted:
04
12
2021
pubmed:
19
1
2022
medline:
8
4
2022
entrez:
18
1
2022
Statut:
ppublish
Résumé
Azurin which is a bacterial secondary metabolite has attracted much attention as potential anticancer agent in recent years. This copper-containing periplasmic redox protein supresses the tumor growth selectively. High-level secretion of proteins into the culture medium offers a significant advantage over periplasmic or cytoplasmic expression. The aim of this study was to investigate the effect of nonionic surfactants on the expression of the Pseudomonas aeruginosa azurin. Different concentrations of Triton X-100 and Tween 80 were used as supplements in growth media and extracellular azurin production was stimulated by both surfactants. According to western blot analysis results, in the presence of Triton X-100, maximum azurin expression level was achieved with 96 h of incubation at 1% concentration, and 48 h at 2% concentration. On the other hand, maximum azurin expression level was achieved in the presence of 1% Tween 80 at 72 h incubation. This study suggested for the first time a high level of azurin secretion from P. aeruginosa in the presence of Triton X-100 or Tween 80, which would be advantageous for the purification procedure.
Identifiants
pubmed: 35039942
doi: 10.1007/s00449-021-02678-5
pii: 10.1007/s00449-021-02678-5
doi:
Substances chimiques
Bacterial Proteins
0
Polysorbates
0
Azurin
12284-43-4
Copper
789U1901C5
Octoxynol
9002-93-1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
553-561Informations de copyright
© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
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