Accurate determination of marker location within whole-brain microscopy images.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
18 01 2022
18 01 2022
Historique:
received:
18
08
2021
accepted:
22
12
2021
entrez:
19
1
2022
pubmed:
20
1
2022
medline:
25
2
2022
Statut:
epublish
Résumé
High-resolution whole-brain microscopy provides a means for post hoc determination of the location of implanted devices and labelled cell populations that are necessary to interpret in vivo experiments designed to understand brain function. Here we have developed two plugins (brainreg and brainreg-segment) for the Python-based image viewer napari, to accurately map any object in a common coordinate space. We analysed the position of dye-labelled electrode tracks and two-photon imaged cell populations expressing fluorescent proteins. The precise location of probes and cells were physiologically interrogated and revealed accurate segmentation with near-cellular resolution.
Identifiants
pubmed: 35042882
doi: 10.1038/s41598-021-04676-9
pii: 10.1038/s41598-021-04676-9
pmc: PMC8766598
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
867Subventions
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : Gatsby Charitable Foundation
ID : GAT3361
Organisme : Wellcome
ID : 090843/F/09/Z
Organisme : Wellcome Trust
ID : 214352/Z/18/Z
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 214333/Z/18/Z
Pays : United Kingdom
Informations de copyright
© 2022. The Author(s).
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