Label-free magnetic nanoparticles-based electrochemical immunosensor for atrazine detection.
Atrazine
Highly porous gold
Label-free electrochemical immunosensor
Magnetic nanoparticles
Prussian blue
Journal
Analytical and bioanalytical chemistry
ISSN: 1618-2650
Titre abrégé: Anal Bioanal Chem
Pays: Germany
ID NLM: 101134327
Informations de publication
Date de publication:
Mar 2022
Mar 2022
Historique:
received:
12
10
2021
accepted:
07
12
2021
revised:
29
11
2021
pubmed:
20
1
2022
medline:
1
4
2022
entrez:
19
1
2022
Statut:
ppublish
Résumé
This work presents the realization of a label-free electrochemical immunosensor for the quick, cheap, and straightforward determination of atrazine. This biodevice is based on developing a technological platform where a gold screen printed electrode (Au-SPE) surface was modified by the electrodeposition of a highly porous gold layer. As an internal probe redox, a Prussian Blue thin layer (PB) was then electrosynthetized onto the modified Au-SPE. Atrazine antibody (Ab-ATZ) was immobilized using G protein-functionalized magnetic nanoparticles (MNPs@protG) to ensure the correct orientation of the antibody to enhance the immunoaffinity. Under optimum experimental conditions, the electrochemical characterization of the developed immunosensor displays a linearity range towards atrazine within 0.05-1.5 ng/mL, a LOD of 0.011 ng/mL good reproducibility and stability. The immunosensor was tested in the analysis of spiked drinking water samples with a mean recovery ranging from 95.7 to 108.4%. The overall good analytical performances of this immunodevice suggest its application for the screening and monitoring of atrazine in real matrices.
Identifiants
pubmed: 35043261
doi: 10.1007/s00216-021-03838-y
pii: 10.1007/s00216-021-03838-y
doi:
Substances chimiques
Magnetite Nanoparticles
0
Gold
7440-57-5
Atrazine
QJA9M5H4IM
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2055-2064Informations de copyright
© 2021. Springer-Verlag GmbH Germany, part of Springer Nature.
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