Regulation of
3Rs principles
consistency approach
gene expression
next-generation sequencing
targeted LC-MS/MS
tetanus vaccine
Journal
Toxins
ISSN: 2072-6651
Titre abrégé: Toxins (Basel)
Pays: Switzerland
ID NLM: 101530765
Informations de publication
Date de publication:
02 01 2022
02 01 2022
Historique:
received:
29
11
2021
revised:
22
12
2021
accepted:
29
12
2021
entrez:
20
1
2022
pubmed:
21
1
2022
medline:
26
2
2022
Statut:
epublish
Résumé
Ensuring consistency of tetanus neurotoxin (TeNT) production by We applied RNA sequencing (RNA-Seq) to study We identified 1381 time-dependent differentially expressed genes (DEGs) reflecting, among others, changes in growth rate and metabolism. Comparing non-standard versus standard culture conditions identified 82 condition-dependent DEGs, most of which were specific for one condition. The tetanus neurotoxin gene ( Non-standard culture conditions lead to different gene expression responses. The
Sections du résumé
BACKGROUND
Ensuring consistency of tetanus neurotoxin (TeNT) production by
METHODS
We applied RNA sequencing (RNA-Seq) to study
RESULTS
We identified 1381 time-dependent differentially expressed genes (DEGs) reflecting, among others, changes in growth rate and metabolism. Comparing non-standard versus standard culture conditions identified 82 condition-dependent DEGs, most of which were specific for one condition. The tetanus neurotoxin gene (
CONCLUSIONS
Non-standard culture conditions lead to different gene expression responses. The
Identifiants
pubmed: 35051008
pii: toxins14010031
doi: 10.3390/toxins14010031
pmc: PMC8778922
pii:
doi:
Substances chimiques
Neurotoxins
0
Tetanus Toxoid
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
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