A Recombinant System and Reporter Viruses for Papiine Alphaherpesvirus 2.
Gaussia luciferase
Papiine alphaherpesvirus 2
antiviral
cell susceptibility
fosmid
herpesvirus
neutralization
recombineering
reporter virus
transformation-associated recombination
Journal
Viruses
ISSN: 1999-4915
Titre abrégé: Viruses
Pays: Switzerland
ID NLM: 101509722
Informations de publication
Date de publication:
05 01 2022
05 01 2022
Historique:
received:
26
11
2021
revised:
27
12
2021
accepted:
30
12
2021
entrez:
22
1
2022
pubmed:
23
1
2022
medline:
22
2
2022
Statut:
epublish
Résumé
Primate simplex viruses, including Herpes simplex viruses 1 and 2, form a group of closely related herpesviruses, which establish latent infections in neurons of their respective host species. While neuropathogenic infections in their natural hosts are rare, zoonotic transmission of Macacine alphaherpesvirus 1 (McHV1) from macaques to humans is associated with severe disease. Human infections with baboon-derived Papiine alphaherpesvirus 2 (PaHV2) have not been reported, although PaHV2 and McHV1 share several biological properties, including neuropathogenicity in mice. The reasons for potential differences in PaHV2 and McHV1 pathogenicity are presently not understood, and answering these questions will require mutagenic analysis. Here, we report the development of a recombinant system, which allows rescue of recombinant PaHV2. In addition, we used recombineering to generate viruses carrying reporter genes (Gaussia luciferase or enhanced green fluorescent protein), which replicate with similar efficiency as wild-type PaHV2. We demonstrate that these viruses can be used to analyze susceptibility of cells to infection and inhibition of infection by neutralizing antibodies and antiviral compounds. In summary, we created a recombinant system for PaHV2, which in the future will be invaluable for molecular analyses of neuropathogenicity of PaHV2.
Identifiants
pubmed: 35062295
pii: v14010091
doi: 10.3390/v14010091
pmc: PMC8778148
pii:
doi:
Substances chimiques
Antibodies, Viral
0
Antiviral Agents
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
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