Proteomic profiling in cerebral amyloid angiopathy reveals an overlap with CADASIL highlighting accumulation of HTRA1 and its substrates.


Journal

Acta neuropathologica communications
ISSN: 2051-5960
Titre abrégé: Acta Neuropathol Commun
Pays: England
ID NLM: 101610673

Informations de publication

Date de publication:
24 01 2022
Historique:
received: 03 12 2021
accepted: 06 12 2021
entrez: 25 1 2022
pubmed: 26 1 2022
medline: 24 3 2022
Statut: epublish

Résumé

Cerebral amyloid angiopathy (CAA) is an age-related condition and a major cause of intracerebral hemorrhage and cognitive decline that shows close links with Alzheimer's disease (AD). CAA is characterized by the aggregation of amyloid-β (Aβ) peptides and formation of Aβ deposits in the brain vasculature resulting in a disruption of the angioarchitecture. Capillaries are a critical site of Aβ pathology in CAA type 1 and become dysfunctional during disease progression. Here, applying an advanced protocol for the isolation of parenchymal microvessels from post-mortem brain tissue combined with liquid chromatography tandem mass spectrometry (LC-MS/MS), we determined the proteomes of CAA type 1 cases (n = 12) including a patient with hereditary cerebral hemorrhage with amyloidosis-Dutch type (HCHWA-D), and of AD cases without microvascular amyloid pathology (n = 13) in comparison to neurologically healthy controls (n = 12). ELISA measurements revealed microvascular Aβ

Identifiants

pubmed: 35074002
doi: 10.1186/s40478-021-01303-6
pii: 10.1186/s40478-021-01303-6
pmc: PMC8785498
doi:

Substances chimiques

Proteome 0
High-Temperature Requirement A Serine Peptidase 1 EC 3.4.21.-
HTRA1 protein, human EC 3.4.21.-

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

6

Informations de copyright

© 2022. The Author(s).

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Auteurs

Andreas Zellner (A)

Institute for Stroke and Dementia Research (ISD), Klinikum der Universität München, Ludwig-Maximilians-Universität München, Feodor-Lynen-Straße 17, 81377, Munich, Germany.
Chair of Proteomics and Bioanalytics, Technical University of Munich (TUM), Freising, Germany.
Research Group Neurohomeostasis, Department of Psychiatry and Psychotherapy, University of Bonn, Bonn, Germany.

Stephan A Müller (SA)

German Center for Neurodegenerative Diseases (DZNE), Munich, Germany.
Neuroproteomics, School of Medicine, Klinikum rechts der Isar, Technische Universität München, Munich, Germany.

Barbara Lindner (B)

Institute for Stroke and Dementia Research (ISD), Klinikum der Universität München, Ludwig-Maximilians-Universität München, Feodor-Lynen-Straße 17, 81377, Munich, Germany.

Nathalie Beaufort (N)

Institute for Stroke and Dementia Research (ISD), Klinikum der Universität München, Ludwig-Maximilians-Universität München, Feodor-Lynen-Straße 17, 81377, Munich, Germany.

Annemieke J M Rozemuller (AJM)

Department of Pathology, Amsterdam Neuroscience, VU University Medical Center, Amsterdam, The Netherlands.

Thomas Arzberger (T)

Department of Psychiatry and Psychotherapy, Klinikum der Universität München, Ludwig-Maximilians-Universität München, Munich, Germany.

Nils C Gassen (NC)

Research Group Neurohomeostasis, Department of Psychiatry and Psychotherapy, University of Bonn, Bonn, Germany.

Stefan F Lichtenthaler (SF)

German Center for Neurodegenerative Diseases (DZNE), Munich, Germany.
Neuroproteomics, School of Medicine, Klinikum rechts der Isar, Technische Universität München, Munich, Germany.
Munich Cluster for Systems Neurology (SyNergy), Munich, Germany.

Bernhard Kuster (B)

Chair of Proteomics and Bioanalytics, Technical University of Munich (TUM), Freising, Germany.

Christof Haffner (C)

Institute for Stroke and Dementia Research (ISD), Klinikum der Universität München, Ludwig-Maximilians-Universität München, Feodor-Lynen-Straße 17, 81377, Munich, Germany. christof.haffner@tum.de.
Department of Psychiatry and Psychotherapy, School of Medicine, Klinikum rechts der Isar, Technische Universität München, Ismaninger Str. 22, 81675, Munich, Germany. christof.haffner@tum.de.

Martin Dichgans (M)

Institute for Stroke and Dementia Research (ISD), Klinikum der Universität München, Ludwig-Maximilians-Universität München, Feodor-Lynen-Straße 17, 81377, Munich, Germany. martin.dichgans@med.uni-muenchen.de.
German Center for Neurodegenerative Diseases (DZNE), Munich, Germany. martin.dichgans@med.uni-muenchen.de.
Munich Cluster for Systems Neurology (SyNergy), Munich, Germany. martin.dichgans@med.uni-muenchen.de.

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