Identification of an allatostatin C signaling system in mollusc Aplysia.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
24 01 2022
24 01 2022
Historique:
received:
06
08
2021
accepted:
06
01
2022
entrez:
25
1
2022
pubmed:
26
1
2022
medline:
8
3
2022
Statut:
epublish
Résumé
Neuropeptides, as pervasive intercellular signaling molecules in the CNS, modulate a variety of behavioral systems in both protostomes and deuterostomes. Allatostatins are neuropeptides in arthropods that inhibit the biosynthesis of juvenile hormones. Based on amino acid sequences, they are divided into three different types in arthropods: allatostatin A, allatostatin B, allatostatin C. Allatostatin C (AstC) was first isolated from Manduca sexta, and it has an important conserved feature of a disulfide bridge formed by two cysteine residues. Moreover, AstC appears to be the ortholog of mammalian somatostatin, and it has functions in common with somatostatin, such as modulating feeding behaviors. The AstC signaling system has been widely studied in arthropods, but minimally studied in molluscs. In this study, we seek to identify the AstC signaling system in the marine mollusc Aplysia californica. We cloned the AstC precursor from the cDNA of Aplysia. We predicted a 15-amino acid peptide with a disulfide bridge, i.e., AstC, using NeuroPred. We then cloned two putative allatostatin C-like receptors and through NCBI Conserved Domain Search we found that they belonged to the G protein-coupled receptor (GPCR) family. In addition, using an inositol monophosphate 1 (IP1) accumulation assay, we showed that Aplysia AstC could activate one of the putative receptors, i.e., the AstC-R, at the lowest EC
Identifiants
pubmed: 35075137
doi: 10.1038/s41598-022-05071-8
pii: 10.1038/s41598-022-05071-8
pmc: PMC8786951
doi:
Substances chimiques
Neuropeptides
0
allatostatin
110119-33-0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1213Subventions
Organisme : National Natural Science Foundation of China
ID : 32100816
Organisme : National Natural Science Foundation of China
ID : 62050071
Organisme : National Natural Science Foundation of China
ID : 31861143036
Organisme : Jiangsu Science and Technology Department
ID : BK20210183
Organisme : Israel Science Foundation
ID : 2396/18
Informations de copyright
© 2022. The Author(s).
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