ERp44 is required for endocardial cushion development by regulating VEGFA secretion in myocardium.
Animals
Cell Proliferation
/ genetics
Endocardial Cushions
/ metabolism
Gene Expression Regulation, Developmental
/ genetics
Heart Defects, Congenital
/ genetics
Membrane Proteins
/ genetics
Mesoderm
/ metabolism
Mice, Knockout
Molecular Chaperones
/ genetics
Myocardium
/ metabolism
Vascular Endothelial Growth Factor A
/ genetics
Journal
Cell proliferation
ISSN: 1365-2184
Titre abrégé: Cell Prolif
Pays: England
ID NLM: 9105195
Informations de publication
Date de publication:
Mar 2022
Mar 2022
Historique:
received:
26
10
2021
accepted:
22
12
2021
pubmed:
29
1
2022
medline:
12
3
2022
entrez:
28
1
2022
Statut:
ppublish
Résumé
Endocardial cushions are precursors of the valve septum complex that separates the four heart chambers. Several genes have been implicated in the development of endocardial cushions. Specifically, ERp44 has been found to play a role in the early secretory pathway, but its function in heart development has not been well studied. In this study, we established conditional and tissue-specific knockout mouse models. The morphology, survival rate, the development of heart and endocardial cushion were under evaluation. The relationship between ERp44 and VEGFA was investigated by transcriptome, qPCR, WB, immunofluorescence and immunohistochemistry. ERp44 knockout (KO) mice were smaller in size, and most mice died during early postnatal life. KO hearts exhibited the typical phenotypes of congenital heart diseases, such as abnormal heart shapes and severe septal and valvular defects. Similar phenotypes were found in cTNT-Cre We demonstrated that ERp44 plays a specific role in heart development. ERp44 contributes to the development of the endocardial cushion by affecting VEGFA-mediated EndMT.
Identifiants
pubmed: 35088919
doi: 10.1111/cpr.13179
pmc: PMC8891561
doi:
Substances chimiques
ERp44 protein, mouse
0
Membrane Proteins
0
Molecular Chaperones
0
Vascular Endothelial Growth Factor A
0
vascular endothelial growth factor A, mouse
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e13179Subventions
Organisme : The First Batch of Clinical Research Project of Air Force Medical University
ID : 2021XB008
Organisme : National Foundation of Sciences and Technology
ID : 31771562
Organisme : National Foundation of Sciences and Technology
ID : 31971051
Organisme : National Key Research and Development Project
ID : 2019YFA0110402
Informations de copyright
© 2022 The Authors. Cell Proliferation published by John Wiley & Sons Ltd.
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