Nondenaturing Solubilization of Inclusion Bodies from Lactic Acid Bacteria.
Inclusion body
Insoluble proteins
L. lactis
Lactobacillus plantarum
Nondenaturing conditions
Solubilization
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2022
2022
Historique:
entrez:
28
1
2022
pubmed:
29
1
2022
medline:
31
3
2022
Statut:
ppublish
Résumé
Since inclusion bodies (IBs) contain an important amount of properly folded and active proteins, their solubilization using nondenaturing conditions to obtain aggregation-prone proteins has gained interest. Through these conditions, the refolding step is no longer required, which avoids the usual protein yield loss after this process. Here, we reveal a simple methodology to obtain pure and active difficult-to-produce proteins using two LPS-free expression systems: Lactococcus lactis and Lactobacillus plantarum. This protocol has proven to be successful to obtain proteins which are labile and prone-to-attach (difficult to be purified from other cytoplasmic proteins) and prone-to-aggregate (difficult to be obtained in their soluble form).
Identifiants
pubmed: 35089570
doi: 10.1007/978-1-0716-1859-2_23
doi:
Substances chimiques
Recombinant Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
389-400Informations de copyright
© 2022. Springer Science+Business Media, LLC, part of Springer Nature.
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