Nondenaturing Solubilization of Inclusion Bodies from Lactic Acid Bacteria.

Inclusion body Insoluble proteins L. lactis Lactobacillus plantarum Nondenaturing conditions Solubilization

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2022
Historique:
entrez: 28 1 2022
pubmed: 29 1 2022
medline: 31 3 2022
Statut: ppublish

Résumé

Since inclusion bodies (IBs) contain an important amount of properly folded and active proteins, their solubilization using nondenaturing conditions to obtain aggregation-prone proteins has gained interest. Through these conditions, the refolding step is no longer required, which avoids the usual protein yield loss after this process. Here, we reveal a simple methodology to obtain pure and active difficult-to-produce proteins using two LPS-free expression systems: Lactococcus lactis and Lactobacillus plantarum. This protocol has proven to be successful to obtain proteins which are labile and prone-to-attach (difficult to be purified from other cytoplasmic proteins) and prone-to-aggregate (difficult to be obtained in their soluble form).

Identifiants

pubmed: 35089570
doi: 10.1007/978-1-0716-1859-2_23
doi:

Substances chimiques

Recombinant Proteins 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

389-400

Informations de copyright

© 2022. Springer Science+Business Media, LLC, part of Springer Nature.

Références

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Auteurs

Laia Gifre-Renom (L)

Department of Ruminant Production, Institut de Recerca i Tecnologia Agroalimentàries (IRTA), Caldes de Montbui, Spain.
Department of Cardiovascular Sciences, Center for Molecular and Vascular Biology, Leuven, Belgium.

Ricardo Baltà-Foix (R)

Department of Ruminant Production, Institut de Recerca i Tecnologia Agroalimentàries (IRTA), Caldes de Montbui, Spain.

Anna Arís (A)

Department of Ruminant Production, Institut de Recerca i Tecnologia Agroalimentàries (IRTA), Caldes de Montbui, Spain. anna.aris@irta.cat.

Elena Garcia-Fruitós (E)

Department of Ruminant Production, Institut de Recerca i Tecnologia Agroalimentàries (IRTA), Caldes de Montbui, Spain. elena.garcia@irta.cat.

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