Morphomolecular and cultural characteristics and host range of Lasiodiplodia theobromae causing stem canker disease in dragon fruit.
Hylocereus polyrhizus
Lasiodiplodia theobromae
multigene phylogeny
Journal
Journal of basic microbiology
ISSN: 1521-4028
Titre abrégé: J Basic Microbiol
Pays: Germany
ID NLM: 8503885
Informations de publication
Date de publication:
Jun 2022
Jun 2022
Historique:
revised:
19
11
2021
received:
20
09
2021
accepted:
15
01
2022
pubmed:
1
2
2022
medline:
3
6
2022
entrez:
31
1
2022
Statut:
ppublish
Résumé
Dragon fruit (Hylocereus polyrhizus) is an economically promising fruit in Bangladesh. The cultivation of dragon fruit has increased fourfold within a decade due to its popularity. Recently, a new disease known as stem canker was reported in some plantations of dragon fruit in Bangladesh, which forced some farmers to abandon their cultivation. This study aimed to explore the morphological, molecular, and cultural characteristics as well as host range of the causal agent associated with this destructive disease. Morphologically similar eight fungal isolates were recovered from eight canker symptomatic dragon fruit stems. Among them, two isolates (namely BU-DLa 01 and BU-DLa 02) were used for a detailed study. Morphological parameters and phylogeny of sequence data of internal transcribed spacer (ITS1, 5.8S rRNA, and ITS2), β-tubulin, and translation elongation factor 1-α identified the isolates as Lasiodiplodia theobromae. The cultural features were studied hinged on the growth of the two isolates on various media, temperature, and pH. Though the mycelial growth of the fungi was supported by all the media tested, potato dextrose agar was the most suitable one for both isolates. The fungi thrived well at a temperature of 25-35°C and 5.5-6.5 pH. Inoculation trials of dragon fruit stem ascertained Koch's postulate. In host range test, the isolates were found pathogenic toward mango, guava, banana, and the fruits of dragon fruit. These data will contribute not only to understanding the biology of L. theobromae as a newly recognized pathogen of H. polyrhizus but also will help in designing a proper management package against this pathogen.
Identifiants
pubmed: 35099080
doi: 10.1002/jobm.202100501
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
689-700Informations de copyright
© 2022 Wiley-VCH GmbH.
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