Differences in thrombin and plasmin generation potential between East African and Western European adults: The role of genetic and non-genetic factors.


Journal

Journal of thrombosis and haemostasis : JTH
ISSN: 1538-7836
Titre abrégé: J Thromb Haemost
Pays: England
ID NLM: 101170508

Informations de publication

Date de publication:
05 2022
Historique:
revised: 19 01 2022
received: 12 09 2021
accepted: 21 01 2022
pubmed: 2 2 2022
medline: 23 4 2022
entrez: 1 2 2022
Statut: ppublish

Résumé

Geographic variability in coagulation across populations and their determinants are poorly understood. To compare thrombin (TG) and plasmin (PG) generation parameters between healthy Tanzanian and Dutch individuals, and to study associations with inflammation and different genetic, host and environmental factors. TG and PG parameters were measured in 313 Tanzanians of African descent living in Tanzania and 392 Dutch of European descent living in the Netherlands and related to results of a dietary questionnaire, circulating inflammatory markers, genotyping, and plasma metabolomics. Tanzanians exhibited an enhanced TG and PG capacity, compared to Dutch participants. A higher proportion of Tanzanians had a TG value in the upper quartile with a PG value in the lower/middle quartile, suggesting a relative pro-coagulant state. Tanzanians also displayed an increased normalized thrombomodulin sensitivity ratio, suggesting reduced sensitivity to protein C. In Tanzanians, PG parameters (lag time and TTP) were associated with seasonality and food-derived plasma metabolites. The Tanzanians had higher concentrations of pro-inflammatory cytokines, which correlated strongly with TG and PG parameters. There was limited overlap in genetic variation associated with TG and PG parameters between the two cohorts. Pathway analysis of genetic variants in the Tanzanian cohort revealed multiple immune pathways that were enriched with TG and PG traits, confirming the importance of co-regulation between coagulation and inflammation. Tanzanians have an enhanced TG and PG potential compared to Dutch individuals, which may relate to differences in inflammation, genetics and diet. These observations highlight the importance of better understanding of the geographic variability in coagulation across populations.

Sections du résumé

BACKGROUND
Geographic variability in coagulation across populations and their determinants are poorly understood.
OBJECTIVE
To compare thrombin (TG) and plasmin (PG) generation parameters between healthy Tanzanian and Dutch individuals, and to study associations with inflammation and different genetic, host and environmental factors.
METHODS
TG and PG parameters were measured in 313 Tanzanians of African descent living in Tanzania and 392 Dutch of European descent living in the Netherlands and related to results of a dietary questionnaire, circulating inflammatory markers, genotyping, and plasma metabolomics.
RESULTS
Tanzanians exhibited an enhanced TG and PG capacity, compared to Dutch participants. A higher proportion of Tanzanians had a TG value in the upper quartile with a PG value in the lower/middle quartile, suggesting a relative pro-coagulant state. Tanzanians also displayed an increased normalized thrombomodulin sensitivity ratio, suggesting reduced sensitivity to protein C. In Tanzanians, PG parameters (lag time and TTP) were associated with seasonality and food-derived plasma metabolites. The Tanzanians had higher concentrations of pro-inflammatory cytokines, which correlated strongly with TG and PG parameters. There was limited overlap in genetic variation associated with TG and PG parameters between the two cohorts. Pathway analysis of genetic variants in the Tanzanian cohort revealed multiple immune pathways that were enriched with TG and PG traits, confirming the importance of co-regulation between coagulation and inflammation.
CONCLUSIONS
Tanzanians have an enhanced TG and PG potential compared to Dutch individuals, which may relate to differences in inflammation, genetics and diet. These observations highlight the importance of better understanding of the geographic variability in coagulation across populations.

Identifiants

pubmed: 35102686
doi: 10.1111/jth.15657
pmc: PMC9305795
pii: S1538-7836(22)00153-2
doi:

Substances chimiques

Thrombin EC 3.4.21.5
Fibrinolysin EC 3.4.21.7

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

1089-1105

Informations de copyright

© 2022 The Authors. Journal of Thrombosis and Haemostasis published by Wiley Periodicals LLC on behalf of International Society on Thrombosis and Haemostasis.

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Auteurs

Godfrey S Temba (GS)

Department of Internal Medicine, Radboudumc Center for Infectious Diseases, Radboud Institute of Health Science (RIHS), Radboud university medical center, Nijmegen, the Netherlands.
Department of Medical Biochemistry and Molecular Biology, Kilimanjaro Christian Medical University College (KCMUCo), Moshi, Tanzania.

Nadira Vadaq (N)

Department of Internal Medicine, Radboudumc Center for Infectious Diseases, Radboud Institute of Health Science (RIHS), Radboud university medical center, Nijmegen, the Netherlands.
Center for Tropical and Infectious Diseases (CENTRID), Faculty of Medicine, Dr. Kariadi Hospital, Diponegoro University, Semarang, Indonesia.

Jun Wan (J)

Synapse Research Institute, Cardiovascular Research Institute Maastricht, Maastricht University Medical Center, Maastricht, the Netherlands.

Vesla Kullaya (V)

Department of Medical Biochemistry and Molecular Biology, Kilimanjaro Christian Medical University College (KCMUCo), Moshi, Tanzania.
Kilimanjaro Clinical Research Institute, Kilimanjaro Christian Medical Center, Moshi, Tanzania.

Dana Huskens (D)

Synapse Research Institute, Cardiovascular Research Institute Maastricht, Maastricht University Medical Center, Maastricht, the Netherlands.

Tal Pecht (T)

Department for Genomics and Immunoregulation, Life & Medical Sciences (LIMES) Institute, University of Bonn, Bonn, Germany.
Systems Medicine, German Center for Neurodegenerative Diseases (DZNE), Bonn, Germany.

Martin Jaeger (M)

Department of Internal Medicine, Radboudumc Center for Infectious Diseases, Radboud Institute of Health Science (RIHS), Radboud university medical center, Nijmegen, the Netherlands.

Collins K Boahen (CK)

Department of Internal Medicine, Radboudumc Center for Infectious Diseases, Radboud Institute of Health Science (RIHS), Radboud university medical center, Nijmegen, the Netherlands.

Vasiliki Matzaraki (V)

Department of Internal Medicine, Radboudumc Center for Infectious Diseases, Radboud Institute of Health Science (RIHS), Radboud university medical center, Nijmegen, the Netherlands.

Wieteke Broeders (W)

Department of Internal Medicine, Radboudumc Center for Infectious Diseases, Radboud Institute of Health Science (RIHS), Radboud university medical center, Nijmegen, the Netherlands.

Leo A B Joosten (LAB)

Department of Internal Medicine, Radboudumc Center for Infectious Diseases, Radboud Institute of Health Science (RIHS), Radboud university medical center, Nijmegen, the Netherlands.

Sultana M H Faradz (SMH)

Division of Human Genetics, Center for Biomedical Research (CEBIOR), Faculty of Medicine, Diponegoro University/Diponegoro National Hospital, Semarang, Indonesia.

Gibson Kibiki (G)

Kilimanjaro Clinical Research Institute, Kilimanjaro Christian Medical Center, Moshi, Tanzania.

Saskia Middeldorp (S)

Department of Internal Medicine, Radboud Institute of Health Science (RIHS), Radboud university medical center, Nijmegen, the Netherlands.

Duccio Cavalieri (D)

Department of Biology, University of Florence, Florence, Italy.

Paolo Lionetti (P)

Departement NEUROFARBA, Meyer Children's Hospital, University of Florence - Gastroenterology and Nutrition Unit, Florence, Italy.

Philip G de Groot (PG)

Synapse Research Institute, Cardiovascular Research Institute Maastricht, Maastricht University Medical Center, Maastricht, the Netherlands.

Joachim L Schultze (JL)

Department for Genomics and Immunoregulation, Life & Medical Sciences (LIMES) Institute, University of Bonn, Bonn, Germany.
Systems Medicine, German Center for Neurodegenerative Diseases (DZNE), Bonn, Germany.
PRECISE Platform for Single Cell Genomics and Epigenomics, German Center for Neurodegenerative Diseases (DZNE) and University of Bonn, Bonn, Germany.

Mihai G Netea (MG)

Department of Internal Medicine, Radboudumc Center for Infectious Diseases, Radboud Institute of Health Science (RIHS), Radboud university medical center, Nijmegen, the Netherlands.
Department for Immunology and Metabolism, Life & Medical Sciences (LIMES) Institute, University of Bonn, Bonn, Germany.

Vinod Kumar (V)

Department of Internal Medicine, Radboudumc Center for Infectious Diseases, Radboud Institute of Health Science (RIHS), Radboud university medical center, Nijmegen, the Netherlands.
Department of Genetics, University Medical Centre Groningen, University of Groningen, Groningen, the Netherlands.

Bas de Laat (B)

Synapse Research Institute, Cardiovascular Research Institute Maastricht, Maastricht University Medical Center, Maastricht, the Netherlands.

Blandina T Mmbaga (BT)

Kilimanjaro Clinical Research Institute, Kilimanjaro Christian Medical Center, Moshi, Tanzania.
Department of Paediatrics, Kilimanjaro Christian Medical University College (KCMUCo), Moshi, Tanzania.

Andre J van der Ven (AJ)

Department of Internal Medicine, Radboudumc Center for Infectious Diseases, Radboud Institute of Health Science (RIHS), Radboud university medical center, Nijmegen, the Netherlands.

Mark Roest (M)

Synapse Research Institute, Cardiovascular Research Institute Maastricht, Maastricht University Medical Center, Maastricht, the Netherlands.

Quirijn de Mast (Q)

Department of Internal Medicine, Radboudumc Center for Infectious Diseases, Radboud Institute of Health Science (RIHS), Radboud university medical center, Nijmegen, the Netherlands.

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