T and B lymphocyte dynamics after genetically-modified pig-to-baboon kidney xenotransplantation with an anti-CD40mAb-based immunosuppressive regimen.
B lymphocytes
Costimulation blockade
Immune monitoring
T lymphocytes
Xenotransplantation
Journal
Transplant immunology
ISSN: 1878-5492
Titre abrégé: Transpl Immunol
Pays: Netherlands
ID NLM: 9309923
Informations de publication
Date de publication:
04 2022
04 2022
Historique:
received:
07
11
2021
accepted:
25
01
2022
pubmed:
4
2
2022
medline:
30
4
2022
entrez:
3
2
2022
Statut:
ppublish
Résumé
The aim was to monitor recovery of T/B lymphocytes in baboons after depletion by anti-thymocyte globulin (ATG) and anti-CD20mAb (Rituximab), followed by pig kidney transplantation and maintenance therapy with an anti-CD40mAb-based regimen. In baboons (n = 14), induction was with ATG and anti-CD20mAb, and maintenance with (i) anti-CD40mAb, (ii) rapamycin, and (iii) methylprednisolone. Follow-up was for 6 months, or until rejection or other complication developed. Baboon blood was collected at intervals to measure T/B cells and subsets by flow cytometry. In a separate study in baboons receiving the same immunosuppressive regimen (n = 10), the populations of T/B lymphocytes in PBMCs, lymph nodes, and spleen were examined. After induction therapy, the total lymphocyte count and the absolute numbers of CD3 ATG and anti-CD20mAb effectively decreased T and B lymphocytes in the blood and, in the presence of anti-CD40mAb maintenance therapy, recovery of these cells was inhibited. The recovery of effector memory CD8
Sections du résumé
BACKGROUND
The aim was to monitor recovery of T/B lymphocytes in baboons after depletion by anti-thymocyte globulin (ATG) and anti-CD20mAb (Rituximab), followed by pig kidney transplantation and maintenance therapy with an anti-CD40mAb-based regimen.
METHODS
In baboons (n = 14), induction was with ATG and anti-CD20mAb, and maintenance with (i) anti-CD40mAb, (ii) rapamycin, and (iii) methylprednisolone. Follow-up was for 6 months, or until rejection or other complication developed. Baboon blood was collected at intervals to measure T/B cells and subsets by flow cytometry. In a separate study in baboons receiving the same immunosuppressive regimen (n = 10), the populations of T/B lymphocytes in PBMCs, lymph nodes, and spleen were examined.
RESULTS
After induction therapy, the total lymphocyte count and the absolute numbers of CD3
CONCLUSIONS
ATG and anti-CD20mAb effectively decreased T and B lymphocytes in the blood and, in the presence of anti-CD40mAb maintenance therapy, recovery of these cells was inhibited. The recovery of effector memory CD8
Identifiants
pubmed: 35114360
pii: S0966-3274(22)00019-3
doi: 10.1016/j.trim.2022.101545
pmc: PMC9395207
mid: NIHMS1779362
pii:
doi:
Substances chimiques
Antibodies
0
Immunosuppressive Agents
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
101545Subventions
Organisme : NIH HHS
ID : P40 OD024628
Pays : United States
Organisme : NIAID NIH HHS
ID : U19 AI090959
Pays : United States
Informations de copyright
Copyright © 2022 Elsevier B.V. All rights reserved.
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