Direct-laser writing for subnanometer focusing and single-molecule imaging.
Animals
CD47 Antigen
/ analysis
COS Cells
Carbocyanines
/ analysis
Chlorocebus aethiops
HEK293 Cells
Humans
Imaging, Three-Dimensional
/ methods
Lasers
Microscopy, Fluorescence
/ methods
Microtubules
/ chemistry
Nanostructures
/ chemistry
Nanotechnology
/ methods
Optical Imaging
/ methods
Polymerization
Reproducibility of Results
Single Molecule Imaging
/ methods
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
03 02 2022
03 02 2022
Historique:
received:
09
08
2020
accepted:
07
12
2021
entrez:
4
2
2022
pubmed:
5
2
2022
medline:
24
2
2022
Statut:
epublish
Résumé
Two-photon direct laser writing is an additive fabrication process that utilizes two-photon absorption of tightly focused femtosecond laser pulses to implement spatially controlled polymerization of a liquid-phase photoresist. Two-photon direct laser writing is capable of nanofabricating arbitrary three-dimensional structures with nanometer accuracy. Here, we explore direct laser writing for high-resolution optical microscopy by fabricating unique 3D optical fiducials for single-molecule tracking and 3D single-molecule localization microscopy. By having control over the position and three-dimensional architecture of the fiducials, we improve axial discrimination and demonstrate isotropic subnanometer 3D focusing (<0.8 nm) over tens of micrometers using a standard inverted microscope. We perform 3D single-molecule acquisitions over cellular volumes, unsupervised data acquisition and live-cell single-particle tracking with nanometer accuracy.
Identifiants
pubmed: 35115532
doi: 10.1038/s41467-022-28219-6
pii: 10.1038/s41467-022-28219-6
pmc: PMC8813935
doi:
Substances chimiques
Alexa Fluor 647
0
CD47 Antigen
0
Carbocyanines
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
647Subventions
Organisme : NCI NIH HHS
ID : P30 CA008748
Pays : United States
Informations de copyright
© 2022. The Author(s).
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