Pyrimidine inhibitors synergize with nucleoside analogues to block SARS-CoV-2.


Journal

Nature
ISSN: 1476-4687
Titre abrégé: Nature
Pays: England
ID NLM: 0410462

Informations de publication

Date de publication:
04 2022
Historique:
received: 22 10 2021
accepted: 26 01 2022
pubmed: 8 2 2022
medline: 16 4 2022
entrez: 7 2 2022
Statut: ppublish

Résumé

The SARS-CoV-2 virus has infected more than 261 million people and has led to more than 5 million deaths in the past year and a half

Identifiants

pubmed: 35130559
doi: 10.1038/s41586-022-04482-x
pii: 10.1038/s41586-022-04482-x
pmc: PMC10377386
mid: NIHMS1876921
doi:

Substances chimiques

Antiviral Agents 0
Hydroxylamines 0
Nucleosides 0
Pyrimidines 0
remdesivir 3QKI37EEHE
Adenosine Monophosphate 415SHH325A
Cytidine 5CSZ8459RP
pyrimidine K8CXK5Q32L
Alanine OF5P57N2ZX
molnupiravir YA84KI1VEW

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

134-140

Subventions

Organisme : NIAID NIH HHS
ID : R01 AI074951
Pays : United States
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : NIAID NIH HHS
ID : T32 AI007524
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI140539
Pays : United States
Organisme : Intramural NIH HHS
ID : ZIA TR000414
Pays : United States
Organisme : NIAID NIH HHS
ID : R21 AI151882
Pays : United States
Organisme : Bill & Melinda Gates Foundation
ID : INV-016638
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI150246
Pays : United States
Organisme : NIAID NIH HHS
ID : R21 AI158134
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI152362
Pays : United States
Organisme : NIAID NIH HHS
ID : R21 AI153480
Pays : United States
Organisme : Bill & Melinda Gates Foundation
ID : INV-018479
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI122749
Pays : United States

Commentaires et corrections

Type : UpdateOf
Type : CommentIn

Informations de copyright

© 2022. The Author(s), under exclusive licence to Springer Nature Limited.

Références

Lu, R. et al. Genomic characterisation and epidemiology of 2019 novel coronavirus: implications for virus origins and receptor binding. Lancet 395, 565–574 (2020).
doi: 10.1016/S0140-6736(20)30251-8 pubmed: 32007145 pmcid: 7159086
Huang, C. et al. Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China. Lancet 395, 497–506 (2020).
pubmed: 31986264 pmcid: 7159299
Huchting, J. Targeting viral genome synthesis as broad-spectrum approach against RNA virus infections. Antivir. Chem. Chemother. 28, 2040206620976786 (2020).
doi: 10.1177/2040206620976786 pubmed: 33297724 pmcid: 7734526
Jordheim, L. P., Durantel, D., Zoulim, F. & Dumontet, C. Advances in the development of nucleoside and nucleotide analogues for cancer and viral diseases. Nat. Rev. Drug Discov. 12, 447–464 (2013).
doi: 10.1038/nrd4010 pubmed: 23722347
Sheahan, T. P. et al. Comparative therapeutic efficacy of remdesivir and combination lopinavir, ritonavir, and interferon beta against MERS-CoV. Nat. Commun. 11, 222 (2020).
doi: 10.1038/s41467-019-13940-6 pubmed: 31924756 pmcid: 6954302
Agostini, M. L. et al. Coronavirus susceptibility to the antiviral remdesivir (GS-5734) is mediated by the viral polymerase and the proofreading exoribonuclease. mBio 9, e00221-18 (2018).
doi: 10.1128/mBio.00221-18 pubmed: 29511076 pmcid: 5844999
Pruijssers, A. J. et al. Remdesivir inhibits SARS-CoV-2 in human lung cells and chimeric SARS-CoV expressing the SARS-CoV-2 RNA polymerase in mice. Cell Rep. 32, 107940 (2020).
doi: 10.1016/j.celrep.2020.107940 pubmed: 32668216 pmcid: 7340027
Sheahan, T. P. et al. Broad-spectrum antiviral GS-5734 inhibits both epidemic and zoonotic coronaviruses. Sci. Transl. Med. 9, eaal3653 (2017).
doi: 10.1126/scitranslmed.aal3653 pubmed: 28659436 pmcid: 5567817
Dittmar, M. et al. Drug repurposing screens reveal cell-type-specific entry pathways and FDA-approved drugs active against SARS-Cov-2. Cell Rep. 35, 108959 (2021).
doi: 10.1016/j.celrep.2021.108959 pubmed: 33811811 pmcid: 7985926
Janes, J. et al. The ReFRAME library as a comprehensive drug repurposing library and its application to the treatment of cryptosporidiosis. Proc. Natl Acad. Sci. USA 115, 10750–10755 (2018).
doi: 10.1073/pnas.1810137115 pubmed: 30282735 pmcid: 6196526
Bakowski, M. A. et al. Drug repurposing screens identify chemical entities for the development of COVID-19 interventions. Nat. Commun. 12, 3309 (2021).
doi: 10.1038/s41467-021-23328-0 pubmed: 34083527 pmcid: 8175350
Riva, L. et al. Discovery of SARS-CoV-2 antiviral drugs through large-scale compound repurposing. Nature 586, 113–119 (2020).
doi: 10.1038/s41586-020-2577-1 pubmed: 32707573 pmcid: 7603405
Yates, M. K. & Seley-Radtke, K. L. The evolution of antiviral nucleoside analogues: a review for chemists and non-chemists. Part II: complex modifications to the nucleoside scaffold. Antiviral Res. 162, 5–21 (2019).
doi: 10.1016/j.antiviral.2018.11.016 pubmed: 30529089
Sheahan, T. P. et al. An orally bioavailable broad-spectrum antiviral inhibits SARS-CoV-2 in human airway epithelial cell cultures and multiple coronaviruses in mice. Sci. Transl. Med. 12, eabb5883 (2020).
doi: 10.1126/scitranslmed.abb5883 pubmed: 32253226
Jordan, P. C. et al. Initiation, extension, and termination of RNA synthesis by a paramyxovirus polymerase. PLoS Pathog. 14, e1006889 (2018).
doi: 10.1371/journal.ppat.1006889 pubmed: 29425244 pmcid: 5823471
Tchesnokov, E. P., Feng, J. Y., Porter, D. P. & Gotte, M. Mechanism of inhibition of Ebola virus RNA-dependent RNA polymerase by remdesivir. Viruses 11, 326 (2019).
doi: 10.3390/v11040326 pmcid: 6520719
Agostini, M. L. et al. Small-molecule antiviral β-D-N
doi: 10.1128/JVI.01348-19 pubmed: 31578288 pmcid: 6880162
Urakova, N. et al. β-D-N
doi: 10.1128/JVI.01965-17 pubmed: 29167335 pmcid: 5774879
Ma, J. & Motsinger-Reif, A. Current methods for quantifying drug synergism. Proteom. Bioinform. 1, 43–48 (2019).
pubmed: 32043089 pmcid: 7010330
Ariav, Y., Ch'ng, J. H., Christofk, H. R., Ron-Harel, N. & Erez, A. Targeting nucleotide metabolism as the nexus of viral infections, cancer, and the immune response. Sci. Adv. 7, eabg6165 (2021).
doi: 10.1126/sciadv.abg6165 pubmed: 34138729 pmcid: 8133749
Wahl, A. et al. SARS-CoV-2 infection is effectively treated and prevented by EIDD-2801. Nature 591, 451–457 (2021).
doi: 10.1038/s41586-021-03312-w pubmed: 33561864 pmcid: 7979515
Cox, R. M., Wolf, J. D. & Plemper, R. K. Therapeutically administered ribonucleoside analogue MK-4482/EIDD-2801 blocks SARS-CoV-2 transmission in ferrets. Nat. Microbiol. 6, 11–18 (2021).
doi: 10.1038/s41564-020-00835-2 pubmed: 33273742
Hou, Y. J. et al. SARS-CoV-2 reverse genetics reveals a variable infection gradient in the respiratory tract. Cell 182, 429–446.e14 (2020).
doi: 10.1016/j.cell.2020.05.042 pubmed: 32526206 pmcid: 7250779
Pizzorno, A. et al. Characterization and treatment of SARS-CoV-2 in nasal and bronchial human airway epithelia. Cell Rep. Med. 1, 100059 (2020).
doi: 10.1016/j.xcrm.2020.100059 pubmed: 32835306 pmcid: 7373044
Tyson, J. R. et al. Improvements to the ARTIC multiplex PCR method for SARS-CoV-2 genome sequencing using nanopore. Preprint at bioRxiv https://doi.org/10.1101/2020.09.04.283077 (2020).
Li, H. Minimap2: pairwise alignment for nucleotide sequences. Bioinformatics 34, 3094–3100 (2018).
doi: 10.1093/bioinformatics/bty191 pubmed: 29750242 pmcid: 6137996
Robinson, J. T. et al. Integrative genomics viewer. Nat. Biotechnol. 29, 24–26 (2011).
doi: 10.1038/nbt.1754 pubmed: 21221095 pmcid: 3346182

Auteurs

David C Schultz (DC)

Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, PA, USA. dschultz@pennmedicine.upenn.edu.

Robert M Johnson (RM)

Department of Microbiology and Immunology, Center for Pathogen Research, University of Maryland School of Medicine, Baltimore, MD, USA.

Kasirajan Ayyanathan (K)

Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, PA, USA.

Jesse Miller (J)

Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, PA, USA.

Kanupriya Whig (K)

Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, PA, USA.

Brinda Kamalia (B)

Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, PA, USA.

Mark Dittmar (M)

Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, PA, USA.

Stuart Weston (S)

Department of Microbiology and Immunology, Center for Pathogen Research, University of Maryland School of Medicine, Baltimore, MD, USA.

Holly L Hammond (HL)

Department of Microbiology and Immunology, Center for Pathogen Research, University of Maryland School of Medicine, Baltimore, MD, USA.

Carly Dillen (C)

Department of Microbiology and Immunology, Center for Pathogen Research, University of Maryland School of Medicine, Baltimore, MD, USA.

Jeremy Ardanuy (J)

Department of Microbiology and Immunology, Center for Pathogen Research, University of Maryland School of Medicine, Baltimore, MD, USA.

Louis Taylor (L)

Department of Microbiology and Immunology, Center for Pathogen Research, University of Maryland School of Medicine, Baltimore, MD, USA.

Jae Seung Lee (JS)

Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, PA, USA.

Minghua Li (M)

Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, PA, USA.

Emily Lee (E)

National Center for Advancing Translational Sciences, National Institutes of Health, Rockville, MD, USA.

Clarissa Shoffler (C)

Metabolomics Core, Penn Cardiovascular Institute, University of Pennsylvania, Philadelphia, PA, USA.

Christopher Petucci (C)

Metabolomics Core, Penn Cardiovascular Institute, University of Pennsylvania, Philadelphia, PA, USA.

Samuel Constant (S)

Epithelix, Geneva, Switzerland.

Marc Ferrer (M)

National Center for Advancing Translational Sciences, National Institutes of Health, Rockville, MD, USA.

Christoph A Thaiss (CA)

Department of Microbiology, University of Pennsylvania, Philadelphia, PA, USA.

Matthew B Frieman (MB)

Department of Microbiology and Immunology, Center for Pathogen Research, University of Maryland School of Medicine, Baltimore, MD, USA. mfrieman@som.umaryland.edu.

Sara Cherry (S)

Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, PA, USA. cherrys@pennmedicine.upenn.edu.
Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, PA, USA. cherrys@pennmedicine.upenn.edu.
Department of Microbiology, University of Pennsylvania, Philadelphia, PA, USA. cherrys@pennmedicine.upenn.edu.

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Classifications MeSH