Olig2 defines a subset of neural stem cells that produce specific olfactory bulb interneuron subtypes in the subventricular zone of adult mice.
Animals
Cell Cycle
/ genetics
Cell Lineage
/ genetics
Cells, Cultured
Female
Gene Knockout Techniques
Interneurons
/ metabolism
Lateral Ventricles
/ embryology
Male
Mice
Mice, Knockout
Neural Stem Cells
/ metabolism
Neurogenesis
/ genetics
Olfactory Bulb
/ embryology
Oligodendrocyte Transcription Factor 2
/ genetics
Signal Transduction
/ genetics
Transcriptome
/ genetics
Adult neurogenesis
Development
Neural stem cell
Olfactory bulb
Regional identity
Journal
Development (Cambridge, England)
ISSN: 1477-9129
Titre abrégé: Development
Pays: England
ID NLM: 8701744
Informations de publication
Date de publication:
01 03 2022
01 03 2022
Historique:
received:
21
07
2021
accepted:
24
01
2022
pubmed:
9
2
2022
medline:
9
3
2022
entrez:
8
2
2022
Statut:
ppublish
Résumé
Distinct neural stem cells (NSCs) reside in different regions of the subventricular zone (SVZ) and generate multiple olfactory bulb (OB) interneuron subtypes in the adult brain. However, the molecular mechanisms underlying such NSC heterogeneity remain largely unknown. Here, we show that the basic helix-loop-helix transcription factor Olig2 defines a subset of NSCs in the early postnatal and adult SVZ. Olig2-expressing NSCs exist broadly but are most enriched in the ventral SVZ along the dorsoventral axis complementary to dorsally enriched Gsx2-expressing NSCs. Comparisons of Olig2-expressing NSCs from early embryonic to adult stages using single cell transcriptomics reveal stepwise developmental changes in their cell cycle and metabolic properties. Genetic studies further show that cross-repression contributes to the mutually exclusive expression of Olig2 and Gsx2 in NSCs/progenitors during embryogenesis, but that their expression is regulated independently from each other in adult NSCs. Finally, lineage-tracing and conditional inactivation studies demonstrate that Olig2 plays an important role in the specification of OB interneuron subtypes. Altogether, our study demonstrates that Olig2 defines a unique subset of adult NSCs enriched in the ventral aspect of the adult SVZ.
Identifiants
pubmed: 35132995
pii: 274286
doi: 10.1242/dev.200028
pmc: PMC8959153
pii:
doi:
Substances chimiques
Olig2 protein, mouse
0
Oligodendrocyte Transcription Factor 2
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NINDS NIH HHS
ID : R01 NS069893
Pays : United States
Informations de copyright
© 2022. Published by The Company of Biologists Ltd.
Déclaration de conflit d'intérêts
Competing interests The authors declare no competing or financial interests.
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