Developing a Microbubble-Based Contrast Agent for Synchrotron Multiple-Image Radiography.
Contrast agents
Microbubbles
Multiple-image radiography
Synchrotron
Ultra-small-angle X-ray scattering
Journal
Molecular imaging and biology
ISSN: 1860-2002
Titre abrégé: Mol Imaging Biol
Pays: United States
ID NLM: 101125610
Informations de publication
Date de publication:
08 2022
08 2022
Historique:
received:
16
07
2021
accepted:
18
01
2022
revised:
04
01
2022
pubmed:
10
2
2022
medline:
22
7
2022
entrez:
9
2
2022
Statut:
ppublish
Résumé
Multiple-image radiography (MIR) is an analyzer-based synchrotron X-ray imaging approach capable of dissociating absorption, refraction, and scattering components of X-ray interaction with the material. It generates additional image contrast mechanisms (besides absorption), especially in the case of soft tissues, while minimizing absorbed radiation dose. Our goal is to develop a contrast agent for MIR using ultrasound microbubbles by carrying out a systematic assessment of size, shell material, and concentration. Microbubbles were synthesized with two different shell materials: phospholipid and polyvinyl-alcohol. Polydisperse perfluorobutane-filled lipid microbubbles were divided into five size groups using centrifugation. Two distributions of air-filled polymer microbubbles were generated: 2-3 µm and 3-4 µm. A subset of polymer microbubbles 3-4 µm had iron oxide nanoparticles incorporated into their shell or coated on their surface. Microbubbles were immobilized in agar with different concentrations: 5 × 10 No difference was detected in absorption or refraction images of all tested microbubbles. Using USAXS, a significant signal increase was observed with lipid microbubbles 6-10 µm at the highest concentration (p = 0.02), but no signal was observed at lower concentrations. These data indicate that lipid microbubbles 6-10 µm are candidates as contrast agents for MIR, specifically for USAXS. A minimum concentration of 5 × 10
Identifiants
pubmed: 35137326
doi: 10.1007/s11307-022-01705-5
pii: 10.1007/s11307-022-01705-5
doi:
Substances chimiques
Contrast Media
0
Lipids
0
Polymers
0
Agar
9002-18-0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
590-599Subventions
Organisme : CIHR
Pays : Canada
Informations de copyright
© 2022. World Molecular Imaging Society.
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