Epithelial LIF signaling limits apoptosis and lung injury during bacterial pneumonia.
LIF
epithelial
innate immunity
lung injury
pneumonia
Journal
American journal of physiology. Lung cellular and molecular physiology
ISSN: 1522-1504
Titre abrégé: Am J Physiol Lung Cell Mol Physiol
Pays: United States
ID NLM: 100901229
Informations de publication
Date de publication:
01 04 2022
01 04 2022
Historique:
pubmed:
10
2
2022
medline:
5
4
2022
entrez:
9
2
2022
Statut:
ppublish
Résumé
During bacterial pneumonia, alveolar epithelial cells are critical for maintaining gas exchange and providing antimicrobial as well as pro-immune properties. We previously demonstrated that leukemia inhibitory factor (LIF), an IL-6 family cytokine, is produced by type II alveolar epithelial cells (ATII) and is critical for tissue protection during bacterial pneumonia. However, the target cells and mechanisms of LIF-mediated protection remain unknown. Here, we demonstrate that antibody-induced LIF blockade remodels the lung epithelial transcriptome in association with increased apoptosis. Based on these data, we performed pneumonia studies using a novel mouse model in which LIFR (the unique receptor for LIF) is absent in lung epithelium. Although LIFR is expressed on the surface of epithelial cells, its absence only minimally contributed to tissue protection during pneumonia. Single-cell RNA-sequencing (scRNAseq) was conducted to identify adult murine lung cell types most prominently expressing
Identifiants
pubmed: 35137631
doi: 10.1152/ajplung.00325.2021
pmc: PMC8957336
doi:
Substances chimiques
Leukemia Inhibitory Factor
0
Banques de données
figshare
['10.6084/m9.figshare.15078915']
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
L550-L563Subventions
Organisme : NHLBI NIH HHS
ID : F32 HL120551
Pays : United States
Organisme : NHLBI NIH HHS
ID : R33 HL137081
Pays : United States
Organisme : NHLBI NIH HHS
ID : K99 HL157555
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL124392
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL111449
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM120060
Pays : United States
Organisme : NHLBI NIH HHS
ID : R35 HL135756
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI115053
Pays : United States
Organisme : NHLBI NIH HHS
ID : T32 HL007035
Pays : United States
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