Translocated Legionella pneumophila small RNAs mimic eukaryotic microRNAs targeting the host immune response.
Bacterial Proteins
/ metabolism
Cell Line
DEAD Box Protein 58
Eukaryota
/ genetics
Extracellular Vesicles
Host-Pathogen Interactions
/ immunology
Humans
Immunity, Innate
Interleukin-1 Receptor-Associated Kinases
Legionella pneumophila
/ metabolism
Legionnaires' Disease
/ immunology
MicroRNAs
/ genetics
Receptors, Immunologic
Signal Transduction
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
09 02 2022
09 02 2022
Historique:
received:
09
11
2020
accepted:
26
01
2022
entrez:
10
2
2022
pubmed:
11
2
2022
medline:
8
3
2022
Statut:
epublish
Résumé
Legionella pneumophila is an intracellular bacterial pathogen that can cause a severe form of pneumonia in humans, a phenotype evolved through interactions with aquatic protozoa in the environment. Here, we show that L. pneumophila uses extracellular vesicles to translocate bacterial small RNAs (sRNAs) into host cells that act on host defence signalling pathways. The bacterial sRNA RsmY binds to the UTR of ddx58 (RIG-I encoding gene) and cRel, while tRNA-Phe binds ddx58 and irak1 collectively reducing expression of RIG-I, IRAK1 and cRel, with subsequent downregulation of IFN-β. Thus, RsmY and tRNA-Phe are bacterial trans-kingdom regulatory RNAs downregulating selected sensor and regulator proteins of the host cell innate immune response. This miRNA-like regulation of the expression of key sensors and regulators of immunity is a feature of L. pneumophila host-pathogen communication and likely represents a general mechanism employed by bacteria that interact with eukaryotic hosts.
Identifiants
pubmed: 35140216
doi: 10.1038/s41467-022-28454-x
pii: 10.1038/s41467-022-28454-x
pmc: PMC8828724
doi:
Substances chimiques
Bacterial Proteins
0
MicroRNAs
0
Receptors, Immunologic
0
IRAK1 protein, human
EC 2.7.11.1
Interleukin-1 Receptor-Associated Kinases
EC 2.7.11.1
RIGI protein, human
EC 3.6.1.-
DEAD Box Protein 58
EC 3.6.4.13
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
762Informations de copyright
© 2022. The Author(s).
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