Cell biological analysis reveals an essential role for Pfcerli2 in erythrocyte invasion by malaria parasites.
Journal
Communications biology
ISSN: 2399-3642
Titre abrégé: Commun Biol
Pays: England
ID NLM: 101719179
Informations de publication
Date de publication:
09 02 2022
09 02 2022
Historique:
received:
17
12
2020
accepted:
23
12
2021
entrez:
10
2
2022
pubmed:
11
2
2022
medline:
5
4
2022
Statut:
epublish
Résumé
Merozoite invasion of host red blood cells (RBCs) is essential for survival of the human malaria parasite Plasmodium falciparum. Proteins involved with RBC binding and invasion are secreted from dual-club shaped organelles at the apical tip of the merozoite called the rhoptries. Here we characterise P. falciparum Cytosolically Exposed Rhoptry Leaflet Interacting protein 2 (PfCERLI2), as a rhoptry bulb protein that is essential for merozoite invasion. Phylogenetic analyses show that cerli2 arose through an ancestral gene duplication of cerli1. We show that PfCERLI2 is essential for blood-stage growth and localises to the cytosolic face of the rhoptry bulb. Inducible knockdown of PfCERLI2 led to a proportion of merozoites failing to invade and was associated with elongation of the rhoptry organelle during merozoite development and inhibition of rhoptry antigen processing. These findings identify PfCERLI2 as a protein that has key roles in rhoptry biology during merozoite invasion.
Identifiants
pubmed: 35140336
doi: 10.1038/s42003-022-03020-9
pii: 10.1038/s42003-022-03020-9
pmc: PMC8828742
doi:
Substances chimiques
Protozoan Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
121Subventions
Organisme : Department of Health | National Health and Medical Research Council (NHMRC)
ID : 1143974
Informations de copyright
© 2022. The Author(s).
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