miR-27a inhibits molecular adhesion between monocytes and human umbilical vein endothelial cells; systemic approach.
Adhesion
HUVEC cells
JAM-B (2)
SELE
SELP
miR-194
miR-27a
Journal
BMC research notes
ISSN: 1756-0500
Titre abrégé: BMC Res Notes
Pays: England
ID NLM: 101462768
Informations de publication
Date de publication:
10 Feb 2022
10 Feb 2022
Historique:
received:
18
10
2021
accepted:
25
01
2022
entrez:
11
2
2022
pubmed:
12
2
2022
medline:
15
2
2022
Statut:
epublish
Résumé
The endothelial cells overexpress the adhesion molecules in the leukocyte diapedesis pathway, developing vessel subendothelial molecular events. In this study, miR-194 and miR-27a were predicted and investigated on the expression of adhesion molecules in HUVEC cells. The SELE, SELP, and JAM-B adhesion molecules involved in the leukocyte tethering were predicted on the GO-enriched gene network. Following transfection of PEI-miRNA particles into HUVEC cells, the SELE, SELP, and JAM-B gene expression levels were evaluated by real-time qPCR. Furthermore, the monocyte-endothelial adhesion was performed using adhesion assay kit. In agreement with the prediction results, the cellular data showed that miR-27a and miR-194 decrease significantly the SELP and JAM-B expression levels in HUVECs (P < 0.05). Moreover, both the miRNAs suppressed the monocyte adhesion to endothelial cells. Since the miR-27a inhibited significantly the monocyte-endothelial adhesion (P = 0.0001) through the suppression of SELP and JAM-B thus it might relate to the leukocyte diapedesis pathway.
Identifiants
pubmed: 35144666
doi: 10.1186/s13104-022-05920-9
pii: 10.1186/s13104-022-05920-9
pmc: PMC8830077
doi:
Substances chimiques
Cell Adhesion Molecules
0
MIRN27 microRNA, human
0
MicroRNAs
0
P-Selectin
0
SELP protein, human
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
31Subventions
Organisme : Iran University of Medical Sciences
ID : 33313
Informations de copyright
© 2022. The Author(s).
Références
Blood. 2012 Feb 9;119(6):1607-16
pubmed: 22184411
BMC Med. 2013 May 01;11:117
pubmed: 23635324
Circ Res. 2016 Feb 19;118(4):703-20
pubmed: 26892968
Mol Biol Cell. 2005 Oct;16(10):4992-5003
pubmed: 16093349
Cardiovasc Res. 2015 Aug 1;107(3):321-30
pubmed: 25990461
J Am Coll Cardiol. 2009 Dec 1;54(23):2129-38
pubmed: 19942084
Nat Rev Immunol. 2007 Jun;7(6):467-77
pubmed: 17525755
J Cell Biochem. 2014 Nov;115(11):1928-36
pubmed: 24905663
Curr Atheroscler Rep. 2013 May;15(5):322
pubmed: 23512606
Mol Med Rep. 2019 Aug;20(2):967-976
pubmed: 31173204
Respir Res. 2020 Jun 10;21(1):144
pubmed: 32522221
Circ Res. 2019 Jan 18;124(2):315-327
pubmed: 30653442
Semin Immunopathol. 2014 Mar;36(2):211-26
pubmed: 24667924
Mol Biol Rep. 2021 Dec;48(12):7913-7920
pubmed: 34652615
Clin Sci (Lond). 2018 Jun 21;132(12):1243-1252
pubmed: 29930142
Int J Mol Sci. 2017 Sep 22;18(10):
pubmed: 28937652
J Exp Med. 1996 Jul 1;184(1):81-92
pubmed: 8691152
Arterioscler Thromb Vasc Biol. 2007 Nov;27(11):2292-301
pubmed: 17673705
Cells. 2018 Mar 26;7(4):
pubmed: 29587442
Theranostics. 2019 Oct 18;9(25):7961-7975
pubmed: 31695809
Free Radic Biol Med. 2013 Sep;64:69-77
pubmed: 23797034
Mol Biol Rep. 2021 Apr;48(4):3503-3513
pubmed: 33860430
Curr Pharm Des. 2011 Dec;17(37):4089-110
pubmed: 22204371
Microvasc Res. 2021 Jul;136:104172
pubmed: 33894273
J Cardiovasc Pharmacol. 2015 Jun;65(6):611-9
pubmed: 25714598
Life Sci. 2021 Aug 15;279:119703
pubmed: 34111458
Biomolecules. 2019 Jul 25;9(8):
pubmed: 31349600
J Neurosci Res. 2019 Oct;97(10):1242-1252
pubmed: 31254290
J Cell Biochem. 2020 Jan;121(1):111-124
pubmed: 31190349
Pharmacol Res. 2013 Sep;75:15-27
pubmed: 23603154
J Lipid Res. 2015 Jan;56(1):38-50
pubmed: 25327529
Circ Res. 2008 Nov 7;103(10):1128-38
pubmed: 18818407
BMC Cardiovasc Disord. 2021 Aug 16;21(1):395
pubmed: 34399692
FASEB J. 2018 Aug;32(8):4070-4084
pubmed: 29565737