Spatial, temporal and molecular dynamics of swine influenza virus-specific CD8 tissue resident memory T cells.


Journal

Mucosal immunology
ISSN: 1935-3456
Titre abrégé: Mucosal Immunol
Pays: United States
ID NLM: 101299742

Informations de publication

Date de publication:
03 2022
Historique:
received: 06 09 2021
accepted: 08 12 2021
revised: 23 11 2021
pubmed: 12 2 2022
medline: 28 4 2022
entrez: 11 2 2022
Statut: ppublish

Résumé

For the first time we have defined naïve, central memory, effector memory and differentiated effector porcine CD8 T cells and analyzed their distribution in lymphoid and respiratory tissues after influenza infection or immunization, using peptide-MHC tetramers of three influenza nucleoprotein (NP) epitopes. The hierarchy of response to the three epitopes changes during the response in different tissues. Most NP-specific CD8 T cells in broncho-alveolar lavage (BAL) and lung are tissue resident memory cells (TRM) that express CD69 and downregulate CD45RA and CCR7. NP-specific cells isolated from BAL express genes characteristic of TRM, but gene expression differs at 7, 21 and 63 days post infection. In all tissues the frequency of NP-specific CD8 cells declines over 63 days almost to background levels but is best maintained in BAL. The kinetic of influenza specific memory CD8 T cell in this natural host species differs from that in small animal models.

Identifiants

pubmed: 35145208
doi: 10.1038/s41385-021-00478-4
pii: S1933-0219(22)00065-4
pmc: PMC9038527
doi:

Substances chimiques

Epitopes 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

428-442

Subventions

Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/L001330/1
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 220295/Z/20/Z
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BBS/E/I/00007036
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/P021336/1
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BBS/E/I/00007031
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BBS/E/I/00007038
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BBS/E/I/00007037
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BBS/E/I/00007039
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BBS/E/I/0000703
Pays : United Kingdom

Informations de copyright

© 2022. The Author(s).

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Auteurs

Veronica Martini (V)

The Pirbright Institute, Pirbright, UK. veronica.martini@pirbright.ac.uk.
Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, UK. veronica.martini@pirbright.ac.uk.
Institute for Research in Biomedicine, Bellinzona, Switzerland. veronica.martini@pirbright.ac.uk.

Matthew Edmans (M)

The Pirbright Institute, Pirbright, UK.

Simon Gubbins (S)

The Pirbright Institute, Pirbright, UK.

Siddharth Jayaraman (S)

The Roslin Institute, Edinburgh, UK.

Basudev Paudyal (B)

The Pirbright Institute, Pirbright, UK.

Sophie Morgan (S)

The Pirbright Institute, Pirbright, UK.

Adam McNee (A)

The Pirbright Institute, Pirbright, UK.

Théo Morin (T)

Division of Infection and Immunity, Cardiff University School of Medicine, Cardiff, UK.

Pramila Rijal (P)

Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, UK.

Wilhelm Gerner (W)

The Pirbright Institute, Pirbright, UK.

Andrew K Sewell (AK)

Division of Infection and Immunity, Cardiff University School of Medicine, Cardiff, UK.

Ryo Inoue (R)

Laboratory of Animal Science, Setsunan University, Osaka, Japan.

Mick Bailey (M)

Bristol Veterinary School, University of Bristol, Langford, UK.

Timothy Connelley (T)

The Roslin Institute, Edinburgh, UK.

Bryan Charleston (B)

The Pirbright Institute, Pirbright, UK.

Alain Townsend (A)

Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, UK.

Peter Beverley (P)

National Heart and Lung Institute, St Mary's Campus, Imperial College, London, UK.

Elma Tchilian (E)

The Pirbright Institute, Pirbright, UK. elma.tchilian@pirbright.ac.uk.

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