VascuViz: a multimodality and multiscale imaging and visualization pipeline for vascular systems biology.
Animals
Brain
/ blood supply
Breast Neoplasms
/ diagnostic imaging
Cerebrovascular Circulation
Contrast Media
Data Visualization
Female
Hemodynamics
Humans
Imaging, Three-Dimensional
/ methods
Magnetic Resonance Imaging
Male
Mice, Inbred Strains
Multimodal Imaging
/ methods
Systems Biology
/ methods
Tomography, X-Ray Computed
Workflow
Journal
Nature methods
ISSN: 1548-7105
Titre abrégé: Nat Methods
Pays: United States
ID NLM: 101215604
Informations de publication
Date de publication:
02 2022
02 2022
Historique:
received:
05
01
2021
accepted:
29
11
2021
entrez:
11
2
2022
pubmed:
12
2
2022
medline:
26
2
2022
Statut:
ppublish
Résumé
Despite advances in imaging, image-based vascular systems biology has remained challenging because blood vessel data are often available only from a single modality or at a given spatial scale, and cross-modality data are difficult to integrate. Therefore, there is an exigent need for a multimodality pipeline that enables ex vivo vascular imaging with magnetic resonance imaging, computed tomography and optical microscopy of the same sample, while permitting imaging with complementary contrast mechanisms from the whole-organ to endothelial cell spatial scales. To achieve this, we developed 'VascuViz'-an easy-to-use method for simultaneous three-dimensional imaging and visualization of the vascular microenvironment using magnetic resonance imaging, computed tomography and optical microscopy in the same intact, unsectioned tissue. The VascuViz workflow permits multimodal imaging with a single labeling step using commercial reagents and is compatible with diverse tissue types and protocols. VascuViz's interdisciplinary utility in conjunction with new data visualization approaches opens up new vistas in image-based vascular systems biology.
Identifiants
pubmed: 35145319
doi: 10.1038/s41592-021-01363-5
pii: 10.1038/s41592-021-01363-5
pmc: PMC8842955
mid: NIHMS1760569
doi:
Substances chimiques
Contrast Media
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
242-254Subventions
Organisme : NCI NIH HHS
ID : R01 CA196701
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA237597
Pays : United States
Organisme : NIDCR NIH HHS
ID : R01 DE027957
Pays : United States
Organisme : NIH HHS
ID : S10 OD012287
Pays : United States
Informations de copyright
© 2022. The Author(s), under exclusive licence to Springer Nature America, Inc.
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