Small RNA pathways in the nematode Ascaris in the absence of piRNAs.
Animals
Argonaute Proteins
/ metabolism
Ascaris
/ genetics
Caenorhabditis elegans
/ genetics
Caenorhabditis elegans Proteins
/ metabolism
Gene Expression Regulation, Developmental
Gene Silencing
Germ Cells
/ metabolism
Phylogeny
RNA, Messenger
/ metabolism
RNA, Small Interfering
/ metabolism
Spermatogenesis
/ genetics
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
11 02 2022
11 02 2022
Historique:
received:
29
07
2021
accepted:
13
01
2022
entrez:
12
2
2022
pubmed:
13
2
2022
medline:
5
3
2022
Statut:
epublish
Résumé
Small RNA pathways play key and diverse regulatory roles in C. elegans, but our understanding of their conservation and contributions in other nematodes is limited. We analyzed small RNA pathways in the divergent parasitic nematode Ascaris. Ascaris has ten Argonautes with five worm-specific Argonautes (WAGOs) that associate with secondary 5'-triphosphate 22-24G-RNAs. These small RNAs target repetitive sequences or mature mRNAs and are similar to the C. elegans mutator, nuclear, and CSR-1 small RNA pathways. Even in the absence of a piRNA pathway, Ascaris CSR-1 may still function to "license" as well as fine-tune or repress gene expression. Ascaris ALG-4 and its associated 26G-RNAs target and likely repress specific mRNAs during testis meiosis. Ascaris WAGO small RNAs demonstrate target plasticity changing their targets between repeats and mRNAs during development. We provide a unique and comprehensive view of mRNA and small RNA expression throughout spermatogenesis. Overall, our study illustrates the conservation, divergence, dynamics, and flexibility of small RNA pathways in nematodes.
Identifiants
pubmed: 35149688
doi: 10.1038/s41467-022-28482-7
pii: 10.1038/s41467-022-28482-7
pmc: PMC8837657
doi:
Substances chimiques
Argonaute Proteins
0
CSR-1 protein, C elegans
0
Caenorhabditis elegans Proteins
0
RNA, Messenger
0
RNA, Small Interfering
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
837Subventions
Organisme : NIAID NIH HHS
ID : R01 AI049558
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI114054
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI155588
Pays : United States
Informations de copyright
© 2022. The Author(s).
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