Water-Soluble Products of Photooxidative Destruction of the Bisretinoid A2E Cause Proteins Modification in the Dark.
A2E
advanced glycation end products
carbonyls
fluorophores
lipofuscin granules
photooxidation
retinal pigment epithelium
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
28 Jan 2022
28 Jan 2022
Historique:
received:
22
12
2021
revised:
21
01
2022
accepted:
26
01
2022
entrez:
15
2
2022
pubmed:
16
2
2022
medline:
17
3
2022
Statut:
epublish
Résumé
Aging of the retina is accompanied by a sharp increase in the content of lipofuscin granules and bisretinoid A2E in the cells of the retinal pigment epithelium (RPE) of the human eye. It is known that A2E can have a toxic effect on RPE cells. However, the specific mechanisms of the toxic effect of A2E are poorly understood. We investigated the effect of the products of photooxidative destruction of A2E on the modification of bovine serum albumin (BSA) and hemoglobin from bovine erythrocytes. A2E was irradiated with a blue light-emitting diode (LED) source (450 nm) or full visible light (400-700 nm) of a halogen lamp, and the resulting water-soluble products of photooxidative destruction were investigated for the content of carbonyl compounds by mass spectrometry and reaction with thiobarbituric acid. It has been shown that water-soluble products formed during A2E photooxidation and containing carbonyl compounds cause modification of serum albumin and hemoglobin, measured by an increase in fluorescence intensity at 440-455 nm. The antiglycation agent aminoguanidine inhibited the process of modification of proteins. It is assumed that water-soluble carbonyl products formed as a result of A2E photodestruction led to the formation of modified proteins, activation of the inflammation process, and, as a consequence, to the progression of various senile eye pathologies.
Identifiants
pubmed: 35163454
pii: ijms23031534
doi: 10.3390/ijms23031534
pmc: PMC8836230
pii:
doi:
Substances chimiques
A2-E (N-retinylidene-N-retinylethanolamine)
0
Guanidines
0
Hemoglobins
0
Retinoids
0
Thiobarbiturates
0
Water
059QF0KO0R
Serum Albumin, Bovine
27432CM55Q
thiobarbituric acid
M1YZW5SS7C
pimagedine
SCQ4EZQ113
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Ministry of Science and Higher Education of Russia
ID : 075-15-2020-795 (local identifier 13.1902.21.0027
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