Plasmid-mediated colistin resistance and ESBL production in Escherichia coli from clinically healthy and sick pigs.
Animals
Anti-Bacterial Agents
/ pharmacology
Colistin
/ pharmacology
Drug Resistance, Multiple, Bacterial
/ genetics
Escherichia coli
/ drug effects
Escherichia coli Infections
/ microbiology
Escherichia coli Proteins
/ biosynthesis
Farms
Feces
/ microbiology
Female
Genes, Bacterial
Genotype
Male
Microbial Sensitivity Tests
Phenotype
Plasmids
Swine
Swine Diseases
/ microbiology
beta-Lactamases
/ biosynthesis
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
14 02 2022
14 02 2022
Historique:
received:
14
06
2021
accepted:
24
01
2022
entrez:
15
2
2022
pubmed:
16
2
2022
medline:
12
3
2022
Statut:
epublish
Résumé
This study aimed to determine the percentage of colistin resistant and ESBL-producing Escherichia coli from clinically sick and healthy pigs and understand the molecular mechanisms underlying colistin resistance and ESBL production. A total of 454 E. coli isolates from healthy pigs (n = 354; piglets, n = 83; fattening pigs, n = 142 and sows, n = 100) and sick pigs (n = 100) were examined for antimicrobial susceptibility, chromosomal and plasmid-mediated colistin resistance mechanisms and ESBL genes. The healthy (41%) and sick pig (73%) isolates were commonly resistant to colistin. Three mcr genes including mcr-1 (10.4%), mcr-2 (1.1%) and mcr-3 (45%) were detected, of which mcr-3 was most frequently detected in the healthy (33%) and sick pig (57%) isolates. Coexistence of mcr-1/mcr-3 and mcr-2/mcr-3 was observed in piglets (23%), fattening pig (3.5%) and sick pig (13%) isolates. Three amino acid substitutions including E106A and G144S in PmrA and V161G in PmrB were observed only in colistin-resistant isolates carrying mcr-3. The percentage of ESBL-producing E. coli was significantly higher in the sick pigs (44%) than the healthy pigs (19.2%) (P = 0.00). The bla
Identifiants
pubmed: 35165337
doi: 10.1038/s41598-022-06415-0
pii: 10.1038/s41598-022-06415-0
pmc: PMC8844364
doi:
Substances chimiques
Anti-Bacterial Agents
0
Escherichia coli Proteins
0
beta-Lactamases
EC 3.5.2.6
Colistin
Z67X93HJG1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2466Informations de copyright
© 2022. The Author(s).
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