Inline monitoring of high cell density cultivation of Scenedesmus rubescens in a mesh ultra-thin layer photobioreactor by photon density wave spectroscopy.
Fiber-optical spectroscopy
Mesh ultra-thin layer photobioreactor
Multiple light scattering
Photon density wave spectroscopy
Process analytical technology
Journal
BMC research notes
ISSN: 1756-0500
Titre abrégé: BMC Res Notes
Pays: England
ID NLM: 101462768
Informations de publication
Date de publication:
15 Feb 2022
15 Feb 2022
Historique:
received:
30
09
2021
accepted:
01
02
2022
entrez:
16
2
2022
pubmed:
17
2
2022
medline:
19
2
2022
Statut:
epublish
Résumé
Due to multiple light scattering that occurs inside and between cells, quantitative optical spectroscopy in turbid biological suspensions is still a major challenge. This includes also optical inline determination of biomass in bioprocessing. Photon Density Wave (PDW) spectroscopy, a technique based on multiple light scattering, enables the independent and absolute determination of optical key parameters of concentrated cell suspensions, which allow to determine biomass during cultivation. A unique reactor type, called "mesh ultra-thin layer photobioreactor" was used to create a highly concentrated algal suspension. PDW spectroscopy measurements were carried out continuously in the reactor without any need of sampling or sample preparation, over 3 weeks, and with 10-min time resolution. Conventional dry matter content and coulter counter measurements have been employed as established offline reference analysis. The PBR allowed peak cell dry weight (CDW) of 33.4 g L
Identifiants
pubmed: 35168633
doi: 10.1186/s13104-022-05943-2
pii: 10.1186/s13104-022-05943-2
pmc: PMC8845379
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
54Subventions
Organisme : Bundesministerium für Bildung und Forschung
ID : 03Z2AN12
Organisme : Land Brandenburg
ID : ILB project number 80139585
Informations de copyright
© 2022. The Author(s).
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