Transcriptome analysis reveals the potential biological function of FSCN1 in HeLa cervical cancer cells.
FSCN1
Gene expression profile
Gene knockdown
RNA-seq
Transcription regulation
Journal
PeerJ
ISSN: 2167-8359
Titre abrégé: PeerJ
Pays: United States
ID NLM: 101603425
Informations de publication
Date de publication:
2022
2022
Historique:
received:
11
05
2021
accepted:
19
01
2022
entrez:
18
2
2022
pubmed:
19
2
2022
medline:
19
2
2022
Statut:
epublish
Résumé
Fascin actin-bundling protein 1 (FSCN1), an actin-bundling protein associated with cell migration and invasion, is highly expressed in various tumor tissues. FSCN1 has also been reported to be a marker of increased invasive potential in cervical cancers. However, the functions of FSCN1 are still not fully understood in cervical cancers. Here, the gene expression profile of HeLa cells transfected with FSCN1 shRNA (shFSCN1) was compared with that of cells transfected with empty vector (shCtrl). The results showed that shFSCN1 extensively affected the transcription level of 5,043 genes in HeLa cells. In particular, Gene Ontology (GO) analysis showed that a large number of upregulated genes were annotated with terms including transcription regulation and DNA binding. The downregulated genes were enriched in some cancer pathways, including angiogenesis and cell adhesion. qPCR validation confirmed that FSCN1 knockdown significantly affected the expression of selected genes in HeLa cells either negatively or positively. Expression analysis in TCGA (The Cancer Genome Atlas) revealed that FSCN1 had negative correlations with several transcription factors and a positive correlation with an angiogenic factor (angiopoietin like 4,
Identifiants
pubmed: 35178306
doi: 10.7717/peerj.12909
pii: 12909
pmc: PMC8817631
doi:
Substances chimiques
MicroRNAs
0
Actins
0
Angiogenesis Inducing Agents
0
Microfilament Proteins
0
FSCN1 protein, human
0
Carrier Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Pagination
e12909Informations de copyright
©2022 Guo et al.
Déclaration de conflit d'intérêts
The authors declare there are no competing interests. Weili Quan is an employee of ABLife BioBigData Institute. Yaxun Wei is an employee of the ABLife Inc.
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