Rapid detection of SARS-CoV-2 variants using allele-specific PCR.
Allele-specific PCR
SARS-CoV-2
Variant assay
Variants of concerns
Whole genome sequencing
Journal
Journal of virological methods
ISSN: 1879-0984
Titre abrégé: J Virol Methods
Pays: Netherlands
ID NLM: 8005839
Informations de publication
Date de publication:
05 2022
05 2022
Historique:
received:
22
11
2021
revised:
12
02
2022
accepted:
15
02
2022
pubmed:
20
2
2022
medline:
16
3
2022
entrez:
19
2
2022
Statut:
ppublish
Résumé
Tracking severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants through whole genome sequencing (WGS) is vital for effective infection control and prevention (IPC) measures, but can be time-consuming and resource-heavy. We describe an in-house validation of an allele-specific polymerase chain reaction (ASP) variant assay to detect variants of concern (VOC). ASP sensitivity for detecting Delta, Alpha and Beta was 99.45 %, 100 %, and 66.67 %, respectively, compared with WGS. Specificity was 100 % in detecting all three VOC. ASP generated results 1.3 days faster compared with WGS. These findings suggest using variant assays such as ASP may enhance epidemiological surveillance and IPC measures.
Identifiants
pubmed: 35182711
pii: S0166-0934(22)00044-1
doi: 10.1016/j.jviromet.2022.114497
pmc: PMC8848537
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
114497Informations de copyright
Copyright © 2022 Elsevier B.V. All rights reserved.
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