Screening of novel fungal Carbohydrate Esterase family 1 enzymes identifies three novel dual feruloyl/acetyl xylan esterases.
acetyl xylan esterase
carbohydrate esterase family 1
feruloyl esterase
fungi
hydroxycinnamic acid
plant biomass
Journal
FEBS letters
ISSN: 1873-3468
Titre abrégé: FEBS Lett
Pays: England
ID NLM: 0155157
Informations de publication
Date de publication:
08 2022
08 2022
Historique:
revised:
09
02
2022
received:
27
12
2021
accepted:
10
02
2022
pubmed:
22
2
2022
medline:
17
8
2022
entrez:
21
2
2022
Statut:
ppublish
Résumé
Feruloyl esterases (FAEs) and acetyl xylan esterases (AXEs) are important enzymes for plant biomass degradation and are both present in Carbohydrate Esterase family 1 (CE1) of the Carbohydrate-Active enZymes database. In this study, ten novel fungal CE1 enzymes from different subfamilies were heterologously produced and screened for their activity towards model and complex plant biomass substrates. CE1_1 enzymes possess AXE activity, while CE1_5 enzymes showed FAE activity. Two enzymes from CE1_2 and one from CE1_5 possess dual feruloyl/acetyl xylan esterase (FXE) activity, showing expansion of substrate specificity. The new FXEs from CE1 can efficiently release both feruloyl and acetyl residues from feruloylated xylan, making them particularly interesting novel components of industrial enzyme cocktails for plant biomass degradation.
Identifiants
pubmed: 35187647
doi: 10.1002/1873-3468.14322
doi:
Substances chimiques
Xylans
0
Esterases
EC 3.1.-
Carboxylic Ester Hydrolases
EC 3.1.1.-
Acetylesterase
EC 3.1.1.6
acetylxylan esterase
EC 3.1.1.72
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1932-1943Informations de copyright
© 2022 The Authors. FEBS Letters published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.
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