Cell-Free Synthesis of Human Endothelin Receptors and Its Application to Ribosome Display.

Cell-Free System Endothelin-1 Humans Phospholipids Protein Engineering / methods Receptor, Endothelin A / biosynthesis Ribosomes

Journal

Analytical chemistry
ISSN: 1520-6882
Titre abrégé: Anal Chem
Pays: United States
ID NLM: 0370536

Informations de publication

Date de publication:
08 03 2022
Historique:
pubmed: 22 2 2022
medline: 15 3 2022
entrez: 21 2 2022
Statut: ppublish

Résumé

Engineering G-protein-coupled receptors (GPCRs) for improved stability or altered function is of great interest, as GPCRs consist of the largest protein family, are involved in many important signaling pathways, and thus, are one of the major drug targets. Here, we report the development of a high-throughput screening method for GPCRs using a reconstituted in vitro transcription-translation (IVTT) system. Human endothelin receptor type-B (ETBR), a class A GPCR that binds endothelin-1 (ET-1), a 21-residue peptide hormone, was synthesized in the presence of nanodisc (ND) composed of a phospholipid, 1-palmitoyl-2-oleoyl-

Identifiants

DOI: 10.1021/acs.analchem.1c04714 PMID: 35188389
pubmed: 35188389
doi: 10.1021/acs.analchem.1c04714
doi:

Substances chimiques

Endothelin-1 0
Phospholipids 0
Receptor, Endothelin A 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

3831-3839

Auteurs

Hiroki Nakai (H)

Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.

Kinuka Isshiki (K)

Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.

Masato Hattori (M)

Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.

Hiromasa Maehira (H)

Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.

Tatsumi Yamaguchi (T)

Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
Earth-Life Science Institute, Tokyo Institute of Technology, Ookayama 2-12-1-i7E-307, Meguro-Ku, Tokyo 152-8550, Japan.

Keiko Masuda (K)

Laboratory for Cell-Free Protein Synthesis, RIKEN Center for Biosystem Dynamics Research (BDR), 6-2-3, Furuedai, Suita, Osaka 565-0874, Japan.

Yoshihiro Shimizu (Y)

Laboratory for Cell-Free Protein Synthesis, RIKEN Center for Biosystem Dynamics Research (BDR), 6-2-3, Furuedai, Suita, Osaka 565-0874, Japan.

Takayoshi Watanabe (T)

School of Materials Science, Japan Advanced Institute of Science and Technology, 1-1 Asahidai, Nomi, Ishikawa 923-1292, Japan.

Takahiro Hohsaka (T)

School of Materials Science, Japan Advanced Institute of Science and Technology, 1-1 Asahidai, Nomi, Ishikawa 923-1292, Japan.

Wataru Shihoya (W)

Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Bunkyo, Tokyo 113-0033, Japan.

Osamu Nureki (O)

Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Bunkyo, Tokyo 113-0033, Japan.

Yasuhiko Kato (Y)

Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.

Hajime Watanabe (H)

Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
ORCID: 0000-0001-9657-6554

Tomoaki Matsuura (T)

Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
Earth-Life Science Institute, Tokyo Institute of Technology, Ookayama 2-12-1-i7E-307, Meguro-Ku, Tokyo 152-8550, Japan.
ORCID: 0000-0003-1015-6781

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Classifications MeSH

questionsmedicales.fr › Cellules › Structures cellulaires › Fractions subcellulaires › Système acellulaire › Cell-Free Synthesis of Human Endothelin...
questionsmedicales.fr › Facteurs biologiques › Protéines et peptides de signalisation intercellulaire › Endothélines › Endothéline-1 › Cell-Free Synthesis of Human Endothelin...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Cell-Free Synthesis of Human Endothelin...
questionsmedicales.fr › Lipides › Lipides membranaires › Phospholipides › Cell-Free Synthesis of Human Endothelin...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Génie génétique › Ingénierie des protéines › Cell-Free Synthesis of Human Endothelin...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Récepteurs de surface cellulaire › Récepteurs peptidiques › Récepteur endothéline › Récepteur de type A de l'endothéline › Cell-Free Synthesis of Human Endothelin...
questionsmedicales.fr › Cellules › Structures cellulaires › Espace intracellulaire › Cytoplasme › Structures cytoplasmiques › Organites › Ribosomes › Cell-Free Synthesis of Human Endothelin...