Carbon-negative production of acetone and isopropanol by gas fermentation at industrial pilot scale.

2-Propanol Acetone Carbon / metabolism Carbon Cycle Fermentation

Journal

Nature biotechnology

ISSN: 1546-1696

Titre abrégé: Nat Biotechnol

Pays: United States

ID NLM: 9604648

Informations de publication

Date de publication:
03 2022

Historique:

received: 06 10 2020

accepted: 09 12 2021

pubmed: 23 2 2022

medline: 20 4 2022

entrez: 22 2 2022

Statut: ppublish

Résumé

Many industrial chemicals that are produced from fossil resources could be manufactured more sustainably through fermentation. Here we describe the development of a carbon-negative fermentation route to producing the industrially important chemicals acetone and isopropanol from abundant, low-cost waste gas feedstocks, such as industrial emissions and syngas. Using a combinatorial pathway library approach, we first mined a historical industrial strain collection for superior enzymes that we used to engineer the autotrophic acetogen Clostridium autoethanogenum. Next, we used omics analysis, kinetic modeling and cell-free prototyping to optimize flux. Finally, we scaled-up our optimized strains for continuous production at rates of up to ~3 g/L/h and ~90% selectivity. Life cycle analysis confirmed a negative carbon footprint for the products. Unlike traditional production processes, which result in release of greenhouse gases, our process fixes carbon. These results show that engineered acetogens enable sustainable, high-efficiency, high-selectivity chemicals production. We expect that our approach can be readily adapted to a wide range of commodity chemicals.

Identifiants

DOI: 10.1038/s41587-021-01195-w PMID: 35190685

pubmed: 35190685

doi: 10.1038/s41587-021-01195-w

pii: 10.1038/s41587-021-01195-w

doi:

Substances chimiques

Acetone 1364PS73AF

Carbon 7440-44-0

2-Propanol ND2M416302

Types de publication

Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

Pagination

335-344

Commentaires et corrections

Type : CommentIn

Informations de copyright

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