The Small DdrR Protein Directly Interacts with the UmuDAb Regulator of the Mutagenic DNA Damage Response in Acinetobacter baumannii.

Acinetobacter baumannii / genetics Bacterial Proteins / metabolism DNA Damage Gene Expression Regulation, Bacterial Mutagens Transcription Factors / metabolism
Acinetobacter baumannii DNA damage DNA repair Induction of gene expression LexA UmuDAb SOS response antibiotic resistance bacteriophage induction

Journal

Journal of bacteriology
ISSN: 1098-5530
Titre abrégé: J Bacteriol
Pays: United States
ID NLM: 2985120R

Informations de publication

Date de publication:
15 03 2022
Historique:
pubmed: 23 2 2022
medline: 22 4 2022
entrez: 22 2 2022
Statut: ppublish

Résumé

Acinetobacter baumannii poses a great threat in health care settings worldwide, with clinical isolates displaying an ever-evolving multidrug resistance. In strains of A. baumannii, expression of multiple error-prone polymerase genes are corepressed by UmuDAb, a member of the LexA superfamily, and a small protein, DdrR. It is currently unknown how DdrR establishes this repression. Here, we used surface plasmon resonance spectrometry to show that DdrR formed a stable complex with the UmuDAb regulator. Our results indicated that the carboxy-terminal dimerization domain of UmuDAb formed the interaction interface with DdrR. Our

Identifiants

DOI: 10.1128/jb.00601-21 PMID: 35191762 PMC: PMC8923225
pubmed: 35191762
doi: 10.1128/jb.00601-21
pmc: PMC8923225
doi:

Substances chimiques

Bacterial Proteins 0
Mutagens 0
Transcription Factors 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

e0060121

Subventions

Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/R017689/1
Pays : United Kingdom

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Auteurs

Anja Pavlin (A)

Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.

Gregor Bajc (G)

Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.

Nadine Fornelos (N)

Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.

Douglas F Browning (DF)

College of Health and Life Sciences, Aston Universitygrid.7273.1, Birmingham, UK.
Institute of Microbiology and Infection, School of Biosciences, University of Birmingham, Birmingham, UK.
ORCID: 0000-0003-4672-3514

Matej Butala (M)

Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.

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Classifications MeSH

questionsmedicales.fr › Bactéries › Proteobacteria › Gammaproteobacteria › Moraxellaceae › Acinetobacter › Acinetobacter baumannii › The Small DdrR Protein Directly Interacts with...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines bactériennes › The Small DdrR Protein Directly Interacts with...
questionsmedicales.fr › Phénomènes génétiques › Altération de l'ADN › The Small DdrR Protein Directly Interacts with...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Régulation de l'expression des gènes bactériens › The Small DdrR Protein Directly Interacts with...
questionsmedicales.fr › Actions chimiques et utilisations › Actions toxiques › Noxas › Mutagènes › The Small DdrR Protein Directly Interacts with...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Facteurs de transcription › The Small DdrR Protein Directly Interacts with...