A novel organophosphate hydrolase from Arthrobacter sp. HM01: Characterization and applications.

Arthrobacter / genetics Organophosphorus Compounds / metabolism Pesticides / chemistry Phosphoric Monoester Hydrolases / chemistry Piperidines
Bioremediation Chemical warfare agents Organophosphorus Pesticides Phosphotriesterase Site-directed mutagenesis

Journal

Bioresource technology
ISSN: 1873-2976
Titre abrégé: Bioresour Technol
Pays: England
ID NLM: 9889523

Informations de publication

Date de publication:
Apr 2022
Historique:
received: 07 01 2022
revised: 14 02 2022
accepted: 16 02 2022
pubmed: 23 2 2022
medline: 15 3 2022
entrez: 22 2 2022
Statut: ppublish

Résumé

Bioremediation systems coupled to efficient microbial enzymes have emerged as an attractive approach for the in-situ removal of hazardous organophosphates (OPs) pesticides from the polluted environment. However, the role of engineered enzymes in OPs-degradation is rarely studied. In this study, the potential OPs-hydrolase (opdH) gene (Arthrobacter sp. HM01) was isolated, cloned, expressed, and purified. The recombinant organophosphate hydrolase (ropdH) was ∼29 kDa; which catalyzed a broad-range of OPs-pesticides in organic-solvent (∼99 % in 30 min), and was found to increase the catalytic efficiency by 10-folds over the native enzyme (k

Identifiants

DOI: 10.1016/j.biortech.2022.126870 PMID: 35192947
pubmed: 35192947
pii: S0960-8524(22)00199-7
doi: 10.1016/j.biortech.2022.126870
pii:
doi:

Substances chimiques

HM01 0
Organophosphorus Compounds 0
Pesticides 0
Piperidines 0
phosphorylphosphatase EC 3.1.3.-
Phosphoric Monoester Hydrolases EC 3.1.3.2

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

126870

Informations de copyright

Copyright © 2022 Elsevier Ltd. All rights reserved.

Auteurs

Himanshu Mali (H)

P. G. Department of Biosciences, UGC-Centre of Advanced Studies, Satellite Campus, Sardar Patel University, Sardar Patel Maidan, Bakrol-Vadtal Road, Bakrol, Gujarat 388 315, India.

Chandni Shah (C)

P. G. Department of Biosciences, UGC-Centre of Advanced Studies, Satellite Campus, Sardar Patel University, Sardar Patel Maidan, Bakrol-Vadtal Road, Bakrol, Gujarat 388 315, India.

Darshan M Rudakiya (DM)

Synergy Cignpost Diagnostics, 3 Mills Studio, London, E3 3DU, United Kingdom.

Darshan H Patel (DH)

Charotar Institute of Paramedical Sciences, Charotar University of Science and Technology, (CHARUSAT), Changa, Gujarat 388421, India.

Ujjval Trivedi (U)

P. G. Department of Biosciences, UGC-Centre of Advanced Studies, Satellite Campus, Sardar Patel University, Sardar Patel Maidan, Bakrol-Vadtal Road, Bakrol, Gujarat 388 315, India.

R B Subramanian (RB)

P. G. Department of Biosciences, UGC-Centre of Advanced Studies, Satellite Campus, Sardar Patel University, Sardar Patel Maidan, Bakrol-Vadtal Road, Bakrol, Gujarat 388 315, India. Electronic address: himanshu0861@gmail.com.

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Classifications MeSH

questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Bâtonnets à Gram positif › Bâtonnets asporogènes à Gram positif › Bâtonnets asporogènes irréguliers à Gram positif › Actinobacteria › Arthrobacter › A novel organophosphate hydrolase from...
questionsmedicales.fr › Composés chimiques organiques › Composés organiques du phosphore › A novel organophosphate hydrolase from...
questionsmedicales.fr › Actions chimiques et utilisations › Actions toxiques › Pesticides › A novel organophosphate hydrolase from...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Esterases › Phosphoric monoester hydrolases › A novel organophosphate hydrolase from...
questionsmedicales.fr › Composés hétérocycliques › Composés hétéromonocycliques › Pipéridines › A novel organophosphate hydrolase from...