Identification and three-dimensional reconstruction of oxytocin receptor expressing astrocytes in the rat and mouse brain.

Animals Astrocytes Central Amygdaloid Nucleus Imaging, Three-Dimensional / methods Immunohistochemistry Mice Rats Receptors, Oxytocin / genetics
Antibody In Situ Hybridization Microscopy Molecular Biology Neuroscience

Journal

STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501

Informations de publication

Date de publication:
18 03 2022
Historique:
entrez: 24 2 2022
pubmed: 25 2 2022
medline: 9 4 2022
Statut: epublish

Résumé

Here, we present a step-by-step protocol for three-dimensional reconstruction of astrocyte morphology, applied to the central amygdala oxytocin receptor-expressing astrocytes. This includes RNAse-free perfusion, combination of RNAscope and immunohistochemistry, and confocal imaging. This protocol provides detailed information about tissue handling and a comprehensive description of the RNAScope technique to label rat and mouse oxytocin receptor mRNA. We also describe three-dimensional reconstruction that allows the assessment of more than 70 different cellular parameters, powerful for studying astrocyte morphology and astrocyte-astrocyte interactions. For complete details on the use and execution of this protocol, please refer to Wahis et al. (2021) and Althammer et al. (2020).

Identifiants

DOI: 10.1016/j.xpro.2022.101160 PMID: 35199030 PMC: PMC8844904
pubmed: 35199030
doi: 10.1016/j.xpro.2022.101160
pii: S2666-1667(22)00040-5
pmc: PMC8844904
doi:

Substances chimiques

Receptors, Oxytocin 0

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

101160

Informations de copyright

© 2022 The Authors.

Déclaration de conflit d'intérêts

The authors declare no conflict of interest.

Références

Acta Neuropathol. 2010 Jan;119(1):7-35
pubmed: 20012068
J Neuroinflammation. 2020 Jul 23;17(1):221
pubmed: 32703230
Nat Neurosci. 2021 Apr;24(4):529-541
pubmed: 33589833
Nat Neurosci. 2021 Mar;24(3):312-325
pubmed: 33589835

Auteurs

Ferdinand Althammer (F)

Center for Neuroinflammation and Cardiometabolic Diseases, Georgia State University, Atlanta, GA 30302-5030, USA.

Eric G Krause (EG)

Department of Pharmacodynamics, College of Pharmacy, University of Florida, Gainesville, FL 32611, USA.
Center of Integrative Cardiovascular and Metabolic Diseases, University of Florida, Gainesville, FL 32611, USA.
McKnight Brain Institute, University of Florida, Gainesville, FL 32611, USA.

Anette D de Kloet (AD)

Department of Physiology and Functional Genomics, College of Medicine, University of Florida, Gainesville, FL 32611, USA.
Center of Integrative Cardiovascular and Metabolic Diseases, University of Florida, Gainesville, FL 32611, USA.
McKnight Brain Institute, University of Florida, Gainesville, FL 32611, USA.

Justin Smith (J)

Department of Pharmacodynamics, College of Pharmacy, University of Florida, Gainesville, FL 32611, USA.
Center of Integrative Cardiovascular and Metabolic Diseases, University of Florida, Gainesville, FL 32611, USA.
McKnight Brain Institute, University of Florida, Gainesville, FL 32611, USA.

Valery Grinevich (V)

Center for Neuroinflammation and Cardiometabolic Diseases, Georgia State University, Atlanta, GA 30302-5030, USA.
Department of Neuropeptide Research for Psychiatry, Central Institute of Mental Health, University of Heidelberg, Mannheim, Germany.

Alexandre Charlet (A)

Centre National de la Recherche Scientifique, University of Strasbourg, Strasbourg, France.
Institute of Cellular and Integrative Neuroscience, INCI CNRS UPR3212 8, Allée du Général Rouvillois 67000 Strasbourg, France.

Javier E Stern (JE)

Center for Neuroinflammation and Cardiometabolic Diseases, Georgia State University, Atlanta, GA 30302-5030, USA.

Articles similaires

Evaluating the efficacy of telesurgery with dual console SSI Mantra Surgical Robotic System: experiment on animal model and clinical trials.

Sudhir Prem Srivastava, Vishwajyoti Pascual Srivastava, Avinesh Singh et al.
1.00
Robotic Surgical Procedures Animals Humans Telemedicine Models, Animal

Odour generalisation and detection dog training.

Lyn Caldicott, Thomas W Pike, Helen E Zulch et al.
1.00
Animals Odorants Dogs Generalization, Psychological Smell

FBXO22 inhibits colitis and colorectal carcinogenesis by regulating the degradation of the S2448-phosphorylated form of mTOR.

Minle Li, Xuan Chen, Pengfei Qu et al.
1.00
Animals TOR Serine-Threonine Kinases Colorectal Neoplasms Colitis Mice

Use of organic material provided by an automatic enrichment device by weaner pigs and its influence on tail lesions.

Philipp Heseker, Jeanette Probst, Stefanie Ammer et al.
1.00
Animals Tail Swine Behavior, Animal Animal Husbandry

Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Identification and three-dimensional...
questionsmedicales.fr › Cellules › Névroglie › Astrocytes › Identification and three-dimensional...
questionsmedicales.fr › Système nerveux › Système nerveux central › Encéphale › Prosencéphale › Télencéphale › Cerveau › Noyaux gris centraux › Amygdale (système limbique) › Noyau central de l'amygdale › Identification and three-dimensional...
questionsmedicales.fr › Sciences de l'information › Méthodologies informatiques › Traitement d'image par ordinateur › Imagerie tridimensionnelle › Identification and three-dimensional...
questionsmedicales.fr › Disciplines des sciences naturelles › Chimie › Biochimie › Immunohistochimie › Identification and three-dimensional...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Identification and three-dimensional...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Rats › Identification and three-dimensional...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Récepteurs de surface cellulaire › Récepteurs peptidiques › Récepteur hormone hypophysaire › Récepteurs à l'ocytocine › Identification and three-dimensional...