The immunophilin CYCLOPHILIN28 affects PSII-LHCII supercomplex assembly and accumulation in Arabidopsis thaliana.
PSII-LHCII supercomplexes
cyclophilin28
immunophilin
peptidyl-prolyl cis/trans isomerase activity
photosynthesis
thylakoid lumen
Journal
Journal of integrative plant biology
ISSN: 1744-7909
Titre abrégé: J Integr Plant Biol
Pays: China (Republic : 1949- )
ID NLM: 101250502
Informations de publication
Date de publication:
Apr 2022
Apr 2022
Historique:
received:
17
01
2022
accepted:
18
02
2022
pubmed:
25
2
2022
medline:
20
4
2022
entrez:
24
2
2022
Statut:
ppublish
Résumé
In plant chloroplasts, photosystem II (PSII) complexes, together with light-harvesting complex II (LHCII), form various PSII-LHCII supercomplexes (SCs). This process likely involves immunophilins, but the underlying regulatory mechanisms are unclear. Here, by comparing Arabidopsis thaliana mutants lacking the chloroplast lumen-localized immunophilin CYCLOPHILIN28 (CYP28) to wild-type and transgenic complemented lines, we determined that CYP28 regulates the assembly and accumulation of PSII-LHCII SCs. Compared to the wild type, cyp28 plants showed accelerated leaf growth, earlier flowering time, and enhanced accumulation of high molecular weight PSII-LHCII SCs under normal light conditions. The lack of CYP28 also significantly affected the electron transport rate. Blue native-polyacrylamide gel electrophoresis analysis revealed more Lhcb6 and less Lhcb4 in M-LHCII-Lhcb4-Lhcb6 complexes in cyp28 versus wild-type plants. Peptidyl-prolyl cis/trans isomerase (PPIase) activity assays revealed that CYP28 exhibits weak PPIase activity and that its K113 and E187 residues are critical for this activity. Mutant analysis suggested that CYP28 may regulate PSII-LHCII SC accumulation by altering the configuration of Lhcb6 via its PPIase activity. Furthermore, the Lhcb6-P139 residue is critical for PSII-LHCII SC assembly and accumulation. Therefore, our findings suggest that CYP28 likely regulates PSII-LHCII SC assembly and accumulation by altering the configuration of P139 of Lhcb6 via its PPIase activity.
Substances chimiques
Light-Harvesting Protein Complexes
0
Photosystem II Protein Complex
0
Immunophilins
EC 5.2.1.8
Peptidylprolyl Isomerase
EC 5.2.1.8
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
915-929Subventions
Organisme : National Natural Science Foundation of China
ID : 31700206
Organisme : Natural Science Basic Research Program of Shaanxi
ID : 2016JM3023
Organisme : Special Scientific Research Project of Education Department of Shaanxi Province
ID : 16JK1792
Informations de copyright
© 2022 Institute of Botany, Chinese Academy of Sciences.
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