Enhanced Blood Plasma Extraction Utilising Viscoelastic Effects in a Serpentine Microchannel.
blood plasma separation
microfluidics
particle migration
viscoelasticity
Journal
Biosensors
ISSN: 2079-6374
Titre abrégé: Biosensors (Basel)
Pays: Switzerland
ID NLM: 101609191
Informations de publication
Date de publication:
14 Feb 2022
14 Feb 2022
Historique:
received:
22
12
2021
revised:
11
02
2022
accepted:
12
02
2022
entrez:
24
2
2022
pubmed:
25
2
2022
medline:
23
3
2022
Statut:
epublish
Résumé
Plasma extraction from blood is essential for diagnosis of many diseases. The critical process of plasma extraction requires removal of blood cells from whole blood. Fluid viscoelasticity promotes cell migration towards the central axis of flow due to differences in normal stress and physical properties of cells. We investigated the effects of altering fluid viscoelasticity on blood plasma extraction in a serpentine microchannel. Poly (ethylene oxide) (PEO) was dissolved into blood to increase its viscoelasticity. The influences of PEO concentration, blood dilution, and flow rate on the performance of cell focusing were examined. We found that focusing performance can be significantly enhanced by adding PEO into blood. The optimal PEO concentration ranged from 100 to 200 ppm with respect to effective blood cell focusing. An optimal flow rate from 1 to 15 µL/min was determined, at least for our experimental setup. Given less than 1% haemolysis was detected at the outlets in all experimental combinations, the proposed microfluidic methodology appears suitable for applications sensitive to haemocompatibility.
Identifiants
pubmed: 35200380
pii: bios12020120
doi: 10.3390/bios12020120
pmc: PMC8869685
pii:
doi:
Substances chimiques
Secologanin Tryptamine Alkaloids
0
Polyethylene Glycols
3WJQ0SDW1A
serpentine (alkaloid)
B503RKE34F
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Griffith University
ID : MHIQ Collaborative Interdisciplinary Grants 2021
Organisme : Australian Research Council
ID : DP180100055;DE210100692; FT180100361
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