Plasma neurofilament light chain as a biomarker for fatal familial insomnia.


Journal

European journal of neurology
ISSN: 1468-1331
Titre abrégé: Eur J Neurol
Pays: England
ID NLM: 9506311

Informations de publication

Date de publication:
06 2022
Historique:
received: 09 02 2022
accepted: 22 02 2022
pubmed: 26 2 2022
medline: 10 5 2022
entrez: 25 2 2022
Statut: ppublish

Résumé

Fatal familial insomnia is a rare hereditary prion disease associated with the D178N-129M PRNP mutation. Early diagnosis is difficult, because the clinical syndrome may overlap with affective disorders. In addition, most known cerebrospinal fluid biomarkers for prion diseases and magnetic resonance imaging do not show a good diagnostic accuracy for fatal familial insomnia. In this context, data on plasma biomarkers are scarce. We analyzed levels of neurofilament light chain, glial fibrillary acidic protein, chitinase-3-like protein 1, calcium-binding protein B, and total tau protein in six serial plasma samples from a patient with fatal familial insomnia. Subsequently, plasma neurofilament light chain was analyzed in n = 25 patients and n = 19 controls. The diagnostic accuracy and associations with disease stage and duration were explored. Among all biomarker candidates in the case study, only neurofilament light chain levels showed a constant evolution and increased over time. They discriminated fatal familial insomnia from controls with an area under the curve of 0.992 (95% confidence interval [CI] = 0.974-1) in the case-control study. Higher concentrations were associated with methionine homozygosity at codon 129 PRNP (p = 0.006), shorter total disease duration (rho = -0.467, p = 0.019, 95% CI = -0.790 to -0.015), and shorter time from sampling to death (rho = -0.467, p = 0.019, 95% CI = -0.773 to -0.019). Plasma neurofilament light chain may be a valuable minimally invasive diagnostic biomarker for fatal familial insomnia after clinical onset. Most important, stage-related increase and association with disease duration indicate potential as a prognostic marker and as a surrogate marker in clinical trials.

Sections du résumé

BACKGROUND AND PURPOSE
Fatal familial insomnia is a rare hereditary prion disease associated with the D178N-129M PRNP mutation. Early diagnosis is difficult, because the clinical syndrome may overlap with affective disorders. In addition, most known cerebrospinal fluid biomarkers for prion diseases and magnetic resonance imaging do not show a good diagnostic accuracy for fatal familial insomnia. In this context, data on plasma biomarkers are scarce.
METHODS
We analyzed levels of neurofilament light chain, glial fibrillary acidic protein, chitinase-3-like protein 1, calcium-binding protein B, and total tau protein in six serial plasma samples from a patient with fatal familial insomnia. Subsequently, plasma neurofilament light chain was analyzed in n = 25 patients and n = 19 controls. The diagnostic accuracy and associations with disease stage and duration were explored.
RESULTS
Among all biomarker candidates in the case study, only neurofilament light chain levels showed a constant evolution and increased over time. They discriminated fatal familial insomnia from controls with an area under the curve of 0.992 (95% confidence interval [CI] = 0.974-1) in the case-control study. Higher concentrations were associated with methionine homozygosity at codon 129 PRNP (p = 0.006), shorter total disease duration (rho = -0.467, p = 0.019, 95% CI = -0.790 to -0.015), and shorter time from sampling to death (rho = -0.467, p = 0.019, 95% CI = -0.773 to -0.019).
CONCLUSIONS
Plasma neurofilament light chain may be a valuable minimally invasive diagnostic biomarker for fatal familial insomnia after clinical onset. Most important, stage-related increase and association with disease duration indicate potential as a prognostic marker and as a surrogate marker in clinical trials.

Identifiants

pubmed: 35212083
doi: 10.1111/ene.15302
doi:

Substances chimiques

Biomarkers 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

1841-1846

Informations de copyright

© 2022 The Authors. European Journal of Neurology published by John Wiley & Sons Ltd on behalf of European Academy of Neurology.

Références

Parchi P, Petersen RB, Chen SG, et al. Molecular pathology of fatal familial insomnia. Brain Pathol. 1998;8:539-548.
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Hermann P, Appleby B, Brandel JP, et al. Biomarkers and diagnostic guidelines for sporadic Creutzfeldt-Jakob disease. Lancet Neurol. 2021;20:235-246.
Schmitz M, Villar-Piqué A, Hermann P, et al. Diagnostic accuracy and prognostic utility of CSF biomarkers in genetic prion diseases. Brain. 2021. doi:10.1093/brain/awab350
Cortelli P, Perani D, Montagna P, et al. Pre-symptomatic diagnosis in fatal familial insomnia: serial neurophysiological and 18FDG-PET studies. Brain. 2006;29:668-675.
Abu-Rumeileh S, Baiardi S, Ladogana A, et al. Comparison between plasma and cerebrospinal fluid biomarkers for the early diagnosis and association with survival in prion disease. J Neurol Neurosurg Psychiatry. 2020;91:1181-1188.
Zerr I, Villar-Piqué A, Hermann P, et al. Diagnostic and prognostic value of plasma neurofilament light and total-tau in sporadic Creutzfeldt-Jakob disease. Alzheimers Res Ther. 2021;13:86.
Thompson AGB, Anastasiadis P, Druyeh R, et al. Evaluation of plasma tau and neurofilament light chain biomarkers in a 12-year clinical cohort of human prion diseases. Mol Psychiatry. 2021;26(10):5955-5966. doi:10.1038/s41380-021-01045-w
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Auteurs

Peter Hermann (P)

Department of Neurology, National Reference Center for CJD Surveillance, Göttingen University Medical Center, Göttingen, Germany.

Sezgi Canaslan (S)

Department of Neurology, National Reference Center for CJD Surveillance, Göttingen University Medical Center, Göttingen, Germany.

Anna Villar-Piqué (A)

Bellvitge Biomedical Research Institute, Hospitalet de Llobregat, Spain.
Network Center for Biomedical Research in Neurodegenerative Diseases, Carlos III Institute of Health, Madrid, Spain.

Timothy Bunck (T)

Department of Neurology, National Reference Center for CJD Surveillance, Göttingen University Medical Center, Göttingen, Germany.

Stefan Goebel (S)

Department of Neurology, National Reference Center for CJD Surveillance, Göttingen University Medical Center, Göttingen, Germany.

Franc Llorens (F)

Department of Neurology, National Reference Center for CJD Surveillance, Göttingen University Medical Center, Göttingen, Germany.
Bellvitge Biomedical Research Institute, Hospitalet de Llobregat, Spain.
Network Center for Biomedical Research in Neurodegenerative Diseases, Carlos III Institute of Health, Madrid, Spain.

Matthias Schmitz (M)

Department of Neurology, National Reference Center for CJD Surveillance, Göttingen University Medical Center, Göttingen, Germany.

Inga Zerr (I)

Department of Neurology, National Reference Center for CJD Surveillance, Göttingen University Medical Center, Göttingen, Germany.
German Center for Neurodegenerative Diseases, Göttingen campus, Göttingen, Germany.

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