FRESH 3D bioprinting a contractile heart tube using human stem cell-derived cardiomyocytes.
FRESH
bioprinting
engineered heart tissue
heart muscle
heart tube
Journal
Biofabrication
ISSN: 1758-5090
Titre abrégé: Biofabrication
Pays: England
ID NLM: 101521964
Informations de publication
Date de publication:
16 03 2022
16 03 2022
Historique:
received:
05
12
2021
accepted:
25
02
2022
pubmed:
26
2
2022
medline:
16
4
2022
entrez:
25
2
2022
Statut:
epublish
Résumé
Here we report the 3D bioprinting of a simplified model of the heart, similar to that observed in embryonic development, where the heart is a linear tube that pumps blood and nutrients to the growing embryo. To this end, we engineered a bioinspired model of the human heart tube using freeform reversible of embedding of suspended hydrogels 3D bioprinting. The 3D bioprinted heart tubes were cellularized using human stem cell-derived cardiomyocytes and cardiac fibroblasts and formed patent, perfusable constructs. Synchronous contractions were achieved ∼3-4 days after fabrication and were maintained for up to a month. Immunofluorescent staining confirmed large, interconnected networks of sarcomeric alpha actinin-positive cardiomyocytes. Electrophysiology was assessed using calcium imaging and demonstrated anisotropic calcium wave propagation along the heart tube with a conduction velocity of ∼5 cm s
Identifiants
pubmed: 35213846
doi: 10.1088/1758-5090/ac58be
pmc: PMC9206822
mid: NIHMS1811816
doi:
Substances chimiques
Hydrogels
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NHLBI NIH HHS
ID : F30 HL154728
Pays : United States
Informations de copyright
© 2022 IOP Publishing Ltd.
Références
Adv Funct Mater. 2021 Feb 10;31(7):
pubmed: 33613150
Cell Stem Cell. 2013 Jan 3;12(1):127-37
pubmed: 23168164
Tissue Eng Part A. 2012 May;18(9-10):910-9
pubmed: 22092279
Biofabrication. 2021 Aug 31;13(4):
pubmed: 34404035
HardwareX. 2021 Apr;9:
pubmed: 34746519
Tissue Eng Part B Rev. 2010 Apr;16(2):169-87
pubmed: 19698068
Cell. 2019 Feb 7;176(4):913-927.e18
pubmed: 30686581
Acta Biomater. 2020 Jan 15;102:220-230
pubmed: 31634626
Proc Natl Acad Sci U S A. 2019 Jan 29;116(5):1543-1548
pubmed: 30635415
Heart. 2002 Feb;87(2):177-84
pubmed: 11796563
Nat Biotechnol. 2007 Sep;25(9):1015-24
pubmed: 17721512
APL Bioeng. 2021 Feb 16;5(1):010904
pubmed: 33644626
Science. 2017 Jun 9;356(6342):1035-1039
pubmed: 28596337
Card Fail Rev. 2017 Apr;3(1):7-11
pubmed: 28785469
Basic Res Cardiol. 2012 Nov;107(6):307
pubmed: 23099820
Proc Natl Acad Sci U S A. 2010 Feb 23;107(8):3329-34
pubmed: 19846783
Proc Natl Acad Sci U S A. 2013 Dec 3;110(49):E4698-707
pubmed: 24255110
Adv Healthc Mater. 2021 Aug;10(15):e2001169
pubmed: 33274834
Lab Chip. 2007 Oct;7(10):1367-70
pubmed: 17896023
Science. 2019 May 3;364(6439):458-464
pubmed: 31048486
Nat Methods. 2014 Aug;11(8):855-60
pubmed: 24930130
ACS Biomater Sci Eng. 2020 Nov 9;6(11):6453-6459
pubmed: 33449644
Circ Res. 2007 May 25;100(10):1503-11
pubmed: 17478728
Nature. 2018 Apr;556(7700):239-243
pubmed: 29618819
Science. 2019 Aug 2;365(6452):482-487
pubmed: 31371612
J Biomed Mater Res A. 2008 Sep;86(3):713-24
pubmed: 18041719
Circulation. 2020 Mar 3;141(9):e139-e596
pubmed: 31992061
Nat Protoc. 2013 Jan;8(1):162-75
pubmed: 23257984
Sci Rep. 2016 Apr 20;6:24726
pubmed: 27095412
PLoS One. 2018 Dec 17;13(12):e0209162
pubmed: 30557409
ACS Biomater Sci Eng. 2017 Nov 13;3(11):3022-3028
pubmed: 31119190
Biomaterials. 2004 Apr;25(9):1639-47
pubmed: 14697865
Circ Res. 2020 Jul 3;127(2):207-224
pubmed: 32228120
Sci Adv. 2015 Oct 23;1(9):e1500758
pubmed: 26601312
Trends Biotechnol. 2020 Aug;38(8):835-845
pubmed: 32673587
PLoS One. 2016 Nov 22;11(11):e0166963
pubmed: 27875570
Expert Rev Cardiovasc Ther. 2012 May;10(5):649-56
pubmed: 22651840
J Mol Cell Cardiol. 2018 May;118:147-158
pubmed: 29604261
Biomed Opt Express. 2015 May 18;6(6):2138-57
pubmed: 26114034
Proc Natl Acad Sci U S A. 2004 Dec 28;101(52):18129-34
pubmed: 15604141
Biomark Insights. 2015 May 27;10(Suppl 1):71-6
pubmed: 26052225
HardwareX. 2018 Apr;3:49-61
pubmed: 30498799
Nature. 2014 Jun 12;510(7504):273-7
pubmed: 24776797
Sci Transl Med. 2021 Jul 21;13(603):
pubmed: 34290054