Molecular and cellular insight into Escherichia coli SslE and its role during biofilm maturation.
Journal
NPJ biofilms and microbiomes
ISSN: 2055-5008
Titre abrégé: NPJ Biofilms Microbiomes
Pays: United States
ID NLM: 101666944
Informations de publication
Date de publication:
25 02 2022
25 02 2022
Historique:
received:
31
03
2021
accepted:
31
01
2022
entrez:
26
2
2022
pubmed:
27
2
2022
medline:
1
4
2022
Statut:
epublish
Résumé
Escherichia coli is a Gram-negative bacterium that colonises the human intestine and virulent strains can cause severe diarrhoeal and extraintestinal diseases. The protein SslE is secreted by a range of pathogenic and commensal E. coli strains. It can degrade mucins in the intestine, promotes biofilm maturation and it is a major determinant of infection in virulent strains, although how it carries out these functions is not well understood. Here, we examine SslE from the commensal E. coli Waksman and BL21 (DE3) strains and the enterotoxigenic H10407 and enteropathogenic E2348/69 strains. We reveal that SslE has a unique and dynamic structure in solution and in response to acidification within mature biofilms it can form a unique aggregate with amyloid-like properties. Furthermore, we show that both SslE monomers and aggregates bind DNA in vitro and co-localise with extracellular DNA (eDNA) in mature biofilms, and SslE aggregates may also associate with cellulose under certain conditions. Our results suggest that interactions between SslE and eDNA are important for biofilm maturation in many E. coli strains and SslE may also be a factor that drives biofilm formation in other SslE-secreting bacteria.
Identifiants
pubmed: 35217675
doi: 10.1038/s41522-022-00272-5
pii: 10.1038/s41522-022-00272-5
pmc: PMC8881592
doi:
Substances chimiques
Escherichia coli Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
9Subventions
Organisme : Wellcome Trust
ID : SBF002/1150
Pays : United Kingdom
Organisme : Cancer Research UK
ID : FC001029
Pays : United Kingdom
Organisme : Medical Research Council
ID : MC_U117533887
Pays : United Kingdom
Organisme : Arthritis Research UK
ID : FC001029
Pays : United Kingdom
Organisme : Wellcome Trust
ID : FC001029
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/R017662/1
Pays : United Kingdom
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : British Heart Foundation
ID : IG/16/2/32273
Pays : United Kingdom
Informations de copyright
© 2022. The Author(s).
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